Static and Dynamic Water Contact Angles, FTIR spectra and in vivo Adhesion data of Electrospun pure DegraPol® Tubes and of Tubes with Incorporated Secretome Harvested from Rabbit Adipose-Derived Stem Cell/Rabbit Achilles Tenocyte Co-Culture
Description
Electrospun Tubes were characterized by static and dynamic water contact angles. We provide raw data for pure DegraPol® tubes and for DegraPol® tubes that were emulsion electrospun (water in oil emulsion) where in the small water droplets secretome was dissolved. The secretome had been previously harvested from a co-culture of rabbit adipose-derived stem cells and rabbit Achilles tenocytes in a ratio of 3:1. In addition, we provide transmission data of Fourier Transformed Infrared Spectroscopy (FTIR) assessed spectra, taken from pure electrospun DegraPol® tubes, of emulsion electrospun DegraPol® tubes with incorporated secretome harvested from the rabbit adipose-derived stem cell/rabbit Achilles tenocyte co-culture and of poly (ethylene glycol). Finally, we present adhesion extent data gathered from a rabbit Achilles tendon full transection model. We assessed how much of the tissue adhered to the surrounding tissue in cross sections of the rabbit Achilles tendons three weeks post operation compared to the entire circumference. The experimental groups were a) a 4-strand Becker suture, b) a 4-strand Becker suture with a pure DegraPol® tube, c) a 4-strand Becker suture, an injection of 50 microliter of a secretome harvested from a 3:1-ratio cell culture of adipose-derived stem cells:rabbit Achilles tenocytes, and a DegraPol® tube releasing such secretome, and d) the non-treated healthy rabbit Achilles tendons as a control.
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Swiss National Science Foundation
310030_197578