PTBP1 enforces ATR-CHK1 signalling determining the potency of CDC7 inhibitors

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Background: CDC7 kinase is crucial for the initiation of DNA replication and is important for replication stress responses. Several ATP-competitive CDC7 inhibitors (CDC7is) are being developed as possible cancer therapeutics. CDC7 inhibition causes a dose-dependent delay in S-phase progression and mild activation of the S-phase checkpoint pathway, which further limits origin firing through ATR activation. Methodology: To identify genes that modulate the cellular responses to CDC7is, we established a CRISPR/Cas9 genome-wide loss-of-function screen. The following data represent the closer investigation of one of these genes, PTBP1, using RNA sequencing, flow cytometry, immunofluorescence analysis and additional techniques as described in the manuscript 'PTBP1 enforces ATR-CHK1 signalling determining the potency of CDC7 inhibitors'. Data: Data represent source data for main figures and supplemental items in support of the manuscript 'PTBP1 enforces ATR-CHK1 signalling determining the potency of CDC7 inhibitors', Göder et al. Data collection, interpretation and findings are addressed in the manuscript.

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