Raw and processed datasets on oxidative stress resistance, peroxide accumulation and fecundity in high-fat diet–fed Drosophila melanogaster subjected to α-lipoic acid and climbing interventions
Description
The survival dataset is organised within the folder “Dataset 1 – Survival following oxidative stress” and contains two subfolders: Group I Survival (unmated flies) and Group II Survival (mated flies). Each folder includes six Excel sheets representing survival data across early, mid and late adulthood for both female and male flies, following a consistent naming convention (e.g., Group I_Early_Female, Group II_Late_Male). Raw survival data were obtained from Drosophila melanogaster exposed to 5% hydrogen peroxide following dietary and exercise interventions. Mortality was recorded at 30-minute intervals until complete lethality, and time of death was documented in hours. Data are organised by treatment condition, vial ID, individual fly ID and time of death. Six treatment conditions were analysed, each comprising five biological replicate vials (V1–V5). In Group I, vials contained five males or five females housed separately, while Group II vials contained five males and five females together, enabling individual-level tracking across sex, mating status, life stage and treatment. The peroxide dataset comprises raw and processed data generated using the FOX assay. The folder includes three Excel files: hydrogen peroxide standard values, Group I peroxide levels and Group II peroxide levels. Standards consist of absorbance values at 560 nm for hydrogen peroxide concentrations from 0 to 20 µM, measured in three technical replicates and used to generate standard curves. Group-specific files contain worksheets with raw absorbance values, peroxide levels in 50 µL homogenate, peroxide levels per fly and protein-normalised peroxide values. Homogenates were prepared from five flies in 500 µL buffer. Data are presented separately for males and females across early, mid and late adulthood, with five biological replicate vials per treatment condition. Protein concentration data were generated using the bicinchoninic acid (BCA) assay and used for peroxide normalisation. The dataset includes protein standard values (0–80 µg/mL BSA) measured in three technical replicates, along with Group I and Group II protein datasets. Files contain raw absorbance values, protein concentrations in 50 µL homogenate and protein levels calculated on a per-fly basis following volume correction. Data are provided separately for males and females across all life-stage fractions with five biological replicates per treatment. The fecundity dataset includes Group I and Group II Excel files corresponding to unmated and mated flies, respectively. Daily egg-laying output of female flies was recorded across five consecutive days (D1–D5). Data are organised by day number and biological replicate vials (V1–V5), with each vial containing two males and two females. Eggs were counted manually following 24-hour transfers to fresh fecundity tubes. The dataset supports calculation of daily, mean and cumulative fecundity while preserving raw egg-count values for independent demographic analysis.
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Steps to reproduce
The experimental design was structured to generate comprehensive datasets capturing oxidative stress resistance, biochemical peroxide accumulation and reproductive output in Drosophila melanogaster under defined physiological and experimental conditions. The study employed a factorial design incorporating mating status, sex, life stage and treatment condition as key variables. Wild-type Canton-S flies were maintained under standard laboratory conditions and synchronised to obtain age-matched cohorts. Following eclosion, flies were transferred to a high-fat diet (HFD) and segregated into two primary experimental groups: Group I (unmated flies), in which males and females were housed separately, and Group II (mated flies), in which males and females were housed together. This grouping enabled assessment of the influence of reproductive status on oxidative stress responses and dataset stratification. To account for age-dependent physiological variation, flies from each group were further divided into three distinct life-stage fractions based on the duration of exposure to experimental conditions: early adulthood (weeks 1–3), mid adulthood (weeks 4–6) and late adulthood (weeks 7–9). Interventions were administered only during the designated life-stage window, while flies were maintained on HFD throughout their lifespan. Within each life-stage fraction, flies were subjected to six experimental treatment conditions: i) HFD alone, (ii) HFD with climbing regimen, (iii) HFD with 2 mM α-lipoic acid (LA), (iv) HFD with 2.5 mM LA, (v) HFD with climbing and 2 mM LA, and (vi) HFD with climbing and 2.5 mM LA. Each treatment condition comprised five independent biological replicate vials. In Group I, each replicate vial contained five male or five female flies housed separately. In Group II, each replicate vial contained five male and five female flies housed together. Three major experimental assays were incorporated into the design. Oxidative stress resistance was assessed using acute hydrogen peroxide challenge, with mortality recorded at fixed time intervals until complete lethality, generating time-to-death survival datasets. Peroxide levels were quantified using the ferrous oxidation–xylenol orange (FOX) assay, supported by hydrogen peroxide standard curves and protein estimation by bicinchoninic acid (BCA) assay, enabling protein-normalised peroxide datasets. Reproductive output was evaluated through daily fecundity measurements recorded over five consecutive days using specialised fecundity tubes. The experimental framework was designed to generate raw, intermediate and processed datasets with clear metadata, consistent naming conventions and replicate structure.