Constitutive Activation and Cytokine Hyperresponsiveness of CD8⁺HLA-DR⁺ T Cells Characterise the Immunometabolic Transition to Prediabetes in Adults with Obesity
Description
Supplementary Files and Source_Data
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This dataset contains supplementary materials and figure source data for a cross-sectional observational study examining CD8⁺HLA-DR⁺ T cell activation across metabolic groups conducted at the Dasman Diabetes Institute, Kuwait City, Kuwait (2018–2020). Ethical approval was obtained from the Kuwait Ministry of Health (IDs: 2017/542 and 2021/1806) and the Dasman Diabetes Institute Ethical Review Committee (ID: RA MoH-2022-002). Supplementary Figure S1 was generated using Illustrae.co based on participant screening and eligibility data extracted from the study database. Participant recruitment used community-based outreach, social media, and word-of-mouth referrals. Eligibility screening, metabolic classification, and ex vivo subset selection criteria are described in the figure legend. Supplementary Table 1 presents anthropometric and metabolic data stratified by biological sex (96 males, 102 females, total n=198). Anthropometric measurements were obtained using calibrated scales and stadiometers following standardised protocols. Metabolic parameters including fasting glucose, lipids, insulin, and C-peptide were measured from fasting blood samples using the cobas b 101 analyser and commercial ELISA kits (Mercodia, Uppsala, Sweden). T cell subset counts were obtained by multi-colour flow cytometry on a BD FACSCanto II instrument. Statistical comparisons between sexes were performed using unpaired t-tests or Mann-Whitney U tests as appropriate in GraphPad Prism version 7.01. Supplementary Table 2 presents associations between CD8⁺HLA-DR⁺ T cell frequency and 38 plasma cytokines and chemokines measured simultaneously using the MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel Premixed 41 Plex (HCYTMAG-60K-PX41, Millipore, USA) on a Luminex-based MILLIPLEX analyser. Plasma was separated from fasting blood samples by centrifugation and stored at −80 °C until analysis. Associations are expressed as regression coefficients with 95% confidence intervals calculated separately for each metabolic group using simple linear regression in GraphPad Prism version 7.01. Source Data provides the participant-level values underlying every panel of Figures 1–4, organised one worksheet per panel with a README describing study groups, variables, units, and statistical methods. Study groups are Lean Healthy (LH, n=88), Healthy Obese/Overweight (HO, n=69), and Prediabetic Obese/Overweight (Pre-OB, n=41). Group-level comparisons (Figures 1, 3, and 4) used one-way ANOVA with Tukey's post hoc test; correlations with glucose-homeostasis markers (Figure 2) used Spearman's rank correlation; and discriminatory performance (Figures 1F and 4A) was assessed by ROC analysis. Source data include T cell subset and activated CD8⁺HLA-DR⁺ frequencies, cardiometabolic and inflammatory markers (blood pressure, heart rate, HDL cholesterol, triglycerides, total cholesterol, CRP, MMP9), C-peptide, HOMA-B%, and PMA/ionomycin-stimulated IFN-γ and TNF-α responses.
Institutions
- Dasman Diabetes InstituteAl Asimah, Kuwait City