Major histocompatibility complex class I during pregnancy and at parturition in the horse placenta
Description
"Materials and methods" folder contains details about RT-qPCR, conditions in which PCR and RT-PCR were performed, protocols for freezing tissues before staining and protocols for IHC-F, IF, IHC-P. "Sequencing results" folder contains sequences of primer products for both RT-PCR and RT-qPCR. "Data" folder contains data from PCR and RT-PCR (PCR and RT-PCR), relative expression of pooled MHC-I and B2M mRNA (RT-qPCR), relative expression of MHC-I protein (Western blots). "Scripts" folder contains scripts for mRNA and protein statistical analyses.
Files
Steps to reproduce
To reproduce the statistical results and figures, you will need to have R installed on your computer (https://www.r-project.org/). The required R packages are listed in the scripts, along with instructions for installing them (in the comments, indicated by the # symbol). When you are ready, load the data in the deposited .csv files using the R command read_csv() (included in the script, but you will have to specify where the files are stored in your computer—see the built-in R help for more details). Once the data is loaded, run the script. All results and figures will be reproduced.
Institutions
- Uniwersytet Warminsko-Mazurski