Long-term CO2 Acclimation Omics Data
Description
N. oceanica CCMP1779 was acclimated using 10% (v/v), 55% (v/v), and 100% CO2 at a flow rate of 3 ml/min. Five consecutive acclimation periods were conducted, each lasting 21 days. Following each round of domestication, algal cells were harvested by centrifugation to generate two parallel samples for whole-genome resequencing and variant detection.A robust, high-CO2-tolerant strain was obtained.
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Steps to reproduce
A total amount of 0.5 μg DNA per sample was used as input material for the DNA library preparations. Sequencing library was generated using Truseq Nano DNA HT Sample Prep Kit (Illumina USA) following manufacturer’s recommendations and index codes were added to each sample. Briefly, genomic DNA sample was fragmented by sonication to a size of 350 bp. Then DNA fragments were endpolished, A-tailed, and ligated withthe full-length adapter for Illumina sequencing, followed by further PCR amplification. After PCR products were purified (AMPure XP system), libraries were analyzed for size distribution by Agilent 2100 Bioanalyzer and quantified by real-time PCR (3nM). Paired-end DNA-seq sequencing library was sequenced with the Illumina NovaSeq system at Shanghai Majorbio Bio-pharm Technology Co.,Ltd.
Institutions
- Zhejiang UniversityZhejiang, Hangzhou