Adaptive laboratory evolution of the hydrocarbonoclastic marine strain Rhodococcus ruber MSA14 to high pyrene concentrations

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Rhodococcus ruber MSA14, isolated from oil-polluted coastal sediments in Baja California, Mexico, was subjected to adaptive laboratory evolution using progressively increasing concentrations of pyrene as the sole carbon and energy source, resulting in the isolation of a high-pyrene-adapted strain, R. ruber AD0911. Whole-genome sequencing and breseq-based variant calling identified 75 mutations in AD0911 relative to MSA14, affecting membrane-associated proteins, transcriptional regulators, cell signaling, and stress-response components. These adaptive mechanisms identified in AD0911 provide new insight into bacterial tolerance evolution under high hydrocarbon stress.

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