Primer sequences and Accession # utilized for real time PCR analysis

Published: 11 June 2026| Version 1 | DOI: 10.17632/8hscz447vk.1
Contributor:
Grace Larsen

Description

All primer sequences were designed to span exon-exon junctions, to minimize the potential of amplifying genomic DNA, using Primer3 software with sequences obtained from GenBank (http://www.ncbi.nlm.nih.gov/). All primer pairs displayed melting curves with a single peak, indicative of a pure, single amplicon, confirmed the specificity of the primers.

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Animal Science

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