Original code: Self-demixing of mRNA copies buffers mRNA:mRNA and mRNA:regulator stoichiometries
Description
Original code related to Cardona et al., Self-demixing of mRNA copies buffers mRNA:mRNA and mRNA:regulator stoichiometries. This repository contains original code and example data to perform: - fluorescent reporters quantification - smFISH images simulation - smFISH images quantification - nanoclusters fitting - condensate quantification - translation rates computing Each folder contains a README.md and a .pdf file explaining how to use the respective code. Source: https://github.com/CardonaEA/code-image-analysis-c-elegans DOI: https://doi.org/10.5281/zenodo.8221981 Reference Cardona et al., Self-demixing of mRNA copies buffers mRNA:mRNA and mRNA:regulator stoichiometries, Cell (2023), https://doi.org/10.1016/j.cell.2023.08.018
Files
Steps to reproduce
See files: How to use - quantification of fluorescent reporter proteins.pdf How to use - quantification of smFISH images.pdf How to use - In Silico simulation of smFISH images.pdf How to use - Nanoclusters curve-fitting.pdf How to use - condensate quantification.pdf How to use - translation rates computing.pdf