Combination of lovastatin and 2-deoxy-D-glucose increases the susceptibility of KRAS mutated human colorectal cancer cells to autophagy inhibition
Description
RAS-driven colorectal cancer relies on glucose metabolism to support uncontrolled growth. However, monotherapy with glycolysis inhibitors like 2-deoxy-D-glucose exhibited limited effectiveness. Recent studies suggest that anti-tumor effects of glycolysis inhibition could be improved by combination treatment with inhibitors of oxidative phosphorylation. Our findings suggest that the combination of lovastatin and 2-deoxy-D-glucose may be a promising regimen concurrently targeting glycolysis, oxidative phosphorylation, and autophagy for the management of RAS-driven colorectal cancers. Supplementary S1 is "The effect of the combination of lovastatin and 2DG, and further inhibition of autophagy on cell proliferation in HCT-8 and CCD-841-CoN cell lines" and Supplementary S2-4 is the non-cropped images obtained from all three Western blotting experiments.
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Figure lengend of Supplementary S1:The effect of the combination of lovastatin and 2DG, and further inhibition of autophagy on cell proliferation in HCT-8 and CCD-841-CoN cell lines. HCT-8 (A) and CCD-841-CoN (C) cells were cultured with different concentrations of lovastatin and 2DG alone or in combination for 72 h and cell proliferation was determined (n=3). HCT-8 (B) and CCD-841-CoN (D) were treated with lovastatin (15 μM) and 2DG (5 mM) alone or in combination for 72 h in the presence or absence of chloroquine (CQ) (6.25 μM) and cell proliferation was determined (n=3).