B-vitamins and selected one-carbon and sulfur-containing metabolites in maternal and cord blood
Description
The purpose of the study was to assess the transfer of B vitamins and key analytes involved in sulfur and one carbon metabolism between the mother and the fetus at the time of delivery. Maternal–fetal transfer was evaluated using the ratio between maternal venous and umbilical cord blood concentrations of the respective analytes. Fetal utilization was estimated by comparing venous and arterial umbilical cord blood concentrations. Data will be published in a peer revied journal, deposition of this data set is mandatory. The study was conducted at the Department of Paediatrics and Inherited Metabolic Disorders, the Department of Gynaecology, Obstetrics and Neonatology, and the Institute of Medical Biochemistry and Laboratory Diagnostics, First Faculty of Medicine, Charles University and General University Hospital in Prague, Czech Republic. All participating women provided written informed consent. The study was approved by the Ethics Committee of the General University Hospital in Prague (approval no. EK VFN 31/21) and was conducted in accordance with the ethical principles of the 1964 Declaration of Helsinki. In total, 130 mother–infant pairs were recruited. The inclusion criterion was a physiological pregnancy completed by an elective cesarean section performed for maternal indications. For all consenting participants, maternal venous blood was collected into lithium heparin tubes prior to peripheral cannula insertion during preoperative preparation for cesarean delivery. Immediately after collection, samples were placed in an ice/water bath, and plasma was separated within 60 minutes. Arterial and venous umbilical cord blood samples (lithium heparin) were obtained at delivery and processed using the same protocol. Analytes were quantified using routine immunochemistry methods, high performance liquid chromatography (HPLC), and LC MS/MS assays, as detailed in the accompanying data table.
Files
Steps to reproduce
For details, see the Description and DATA MODEL DETAILS sheet in the Excel file.
Institutions
- Charles UniversityPrague, Prague