Data for corrections to Das et al., 2018, Cell 173, 1–16

Published: 10 December 2018| Version 1 | DOI: 10.17632/8rrgmgdtxr.1
Contributor:
David Sinclair

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Figure 2C autorad: Western blot of lung and thymus tissue from endothelial-specific SIRT1 knockout mice (ESKO-1,2,3) and wild-type mice (WT-1,2,3) probed with anti-SIRT1 (Sigma) and anti-tubulin antibodies (Sigma). The SIRT1 protein level is reduced in ESKO mice compared to WT mice. Figure 2C autorad long: Western blot of lung tissue from endothelial-specific SIRT1 knockout (ESKO-1,2,3) and wild-type (WT-1,2,3) mice probed with an anti-SIRT1 antibody (Sigma) after longer exposure (compare with Figure 2C autorad). Images from Figure 2C autorad and Figure 2C autorad long exposure were used to generate Fig. 2C. Lung Western Replicates: Western blot of lung tissue from endothelial-specific SIRT1 knockout mice (ESKO-4,5,6) and wild-type mice (WT-4,5,6) probed with anti-SIRT1 (Sigma) and anti-tubulin antibodies (Sigma). Fig. 2D_ESKO_CD31: A transverse quadriceps cross-section (20X) from an endothelial-specific SIRT1 knockout (ESKO) mouse immunostained with rat anti-CD31 (Abcam) and anti-rat Alexa Fluor 488 (Life Technologies) antibodies to visualize muscle capillaries. Fig. 2D_ESKO_Laminin: A transverse quadriceps cross-section (20X) from an endothelial-specific SIRT1 knockout (ESKO) mouse immunostained with rabbit anti-laminin (Sigma) and anti-rabbit Alexa Fluor 594 (Life Technologies) antibodies to visualize basal lamina. Fig. 2D_WT_CD31: A transverse quadriceps cross-section (20X) from a wild-type (WT) mouse immunostained with rat anti-CD31 (Abcam) and anti-rat Alexa Fluor 488 (Life Technologies) antibodies to visualize muscle capillaries. Fig. 2D_WT_Laminin: A transverse quadriceps cross-section (20X) from a wild-type (WT) mouse immunostained with rabbit anti-laminin (Sigma) and anti-rabbit Alexa Fluor 594 (Life Technologies) antibodies to visualize basal lamina. Fig. 2D_Capillary Density: Table showing the quantification of capillary densities in quadricep cross-sections from endothelial-specific SIRT1 knockout (ESKO) and wild-type (WT) mice. ESKO_CD31_Replicate: A transverse quadriceps cross-section (20X) from an endothelial-specific SIRT1 knockout (ESKO) mouse immunostained with rat anti-CD31 (Abcam) and anti-rat Alexa Fluor 488 (Life Technologies) antibodies to visualize muscle capillaries. ESKO_Laminin_Replicate: A transverse quadriceps cross-section (20X) from an endothelial-specific SIRT1 knockout (ESKO) mouse immunostained with rabbit anti-laminin (Sigma) and anti-rabbit Alexa Fluor 594 (Life Technologies) antibodies to visualize the basal lamina. WT_CD31_Replicate: A transverse quadriceps cross-section (20X) from a wild-type (WT) mouse immunostained with rat anti-CD31 (Abcam) and anti-rat Alexa Fluor 488 (Life Technologies) antibodies to visualize muscle capillaries. WT_Laminin_Replicate: A transverse quadriceps cross-section (20X) from a wild-type (WT) mouse immunostained with rabbit anti-laminin (Sigma) and anti-rabbit Alexa Fluor 594 (Life Technologies) antibodies to visualize the basal lamina.

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Please see methods published in Das et al., 2018, Cell 173, 1–16

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Massachusetts Institute of Technology, Harvard Medical School

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