Stability and biocompatibility of a Bionic Eye - Histology and immunohistochemistry data
Published: 21 April 2021| Version 1 | DOI: 10.17632/8rzc45vp77.1
Contributors:
Samuel Eggenberger,
Natalie James,
Cherry Ho,
Steven Eamegdool,
Veronika Tatarinoff,
Naomi Craig,
Barry Gow,
Susan Wan,
Christopher Dodds,
Donna La Hood,
Aaraon Gilmour,
Shannon Donahoe,
Mark Krockenberger,
Krishna Tumuluri,
Melville da Cruz,
John Grigg,
Peter McCluskey,
Nigel Lovell,
Michele Madigan,
Adrian Fung,
Gregg Suaning
Description
Data related to: Implantation and long-term assessment of the stability and biocompatibility of a novel 98 channel suprachoroidal visual prosthesis in sheep
In Eggenberger et al., nine sheep of the Suffolk (N=2) and Dorper (N=7) breeds were implanted in the left eye with a suprachoroidal retinal stimulator (Bionic Eye) for durations up to 100 days. The surgical safety, implant stability and device biocompatibility were assessed.
All procedures were performed conducted in accordance with the Australian Code for the Care and Use of Animals for Experimental Purposes 8th edition (2013) and the ARVO standards for use of animals in ophthalmic research and approved by the Animal Care and Ethics Committee (ACEC) of UNSW Sydney (protocol number 14/155B) .
Animal identifier:
Animals were identified according to the experimental group to which they were assigned (duration of experiment 2 days, 1 month, 2 months and 3 months.
E.g.: 2D#1 was the first animal in the group implanted for a duration of 2 Days. 3M#3 was the third animal in the group implanted for 3 Months.
Heamatoxylin and eosin staining:
Sections were then stained with Harris Hematoxylin (7 min; prior to bluing in running tap water for 4 min) and eosin (4 min).
Immunohistochemistry:
GFAP-Cy3 conjugate (mouse IgG; 1:800); cone photoreceptors: opsin-red/green (opsin R/G) (rabbit IgG; 1:500, 1:1000) (Merck Millipore) and macrophage/microglia: Iba1 (goat IgG; 1:200) (Abcam, USA).
Secondary antibodies to goat IgG, mouse IgG, or rabbit IgG, conjugated with Alexa-Fluor 488, 555, or 647 (Invitrogen, USA).
Nuclei: Hoechst 33258 (1:10,000)
Mounting in glycerol:PBS (80:20)
All images were captured using an LSM 700 Meta Confocal microscope system and ZEN Blue software (Carl Zeiss, Germany). Images were assembled in Photoshop CS software (Adobe Corporation, USA).
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University of Sydney, University of New South Wales
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Applied Sciences
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Implantation and long-term assessment of the stability and biocompatibility of a novel 98 channel suprachoroidal visual prosthesis in sheep
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Authors
Eggenberger, Samuel C. James, Natalie L. Ho, Cherry Eamegdool, Steven S. Tatarinoff, Veronika Craig, Naomi A. Gow, Barry S. Wan, Susan Dodds, Christopher W. D. La Hood, Donna Gilmour, Aaron Donahoe, Shannon L. Krockenberger, Mark Tumuluri, Krishna da Cruz, Melville J. Grigg, John R. McCluskey, Peter Lovell, Nigel H. Madigan, Michele C. Fung, Adrian T. Gregg J. Suaning