Processed antimicrobial and fresh-produce decontamination data supporting a spice-based organic sanitizer study

Description

This dataset contains processed summary data supporting the manuscript “Antimicrobial Efficacy of Spice-Based Organic Sanitizers Formulated from Piper guineense, Xylopia aethiopica, Monodora myristica and Tetrapleura tetraptera Against Foodborne Pathogens, and Their Application on Fresh Produce.” The study evaluated cold ethanolic extracts of Piper guineense (Uziza), Xylopia aethiopica (Uda), Monodora myristica (Ehuru), and Tetrapleura tetraptera (Aidan fruit), individually and in binary, ternary, and quaternary blends, against Escherichia coli, Staphylococcus aureus, and Aspergillus niger. The deposited data include inhibition-zone measurements reported as mean ± standard deviation, minimum inhibitory, bactericidal and fungicidal concentrations (MIC/MBC/MFC), and total viable bacterial counts (TVBC) before and after sanitizer treatment of cucumber and tomato, together with the reported percentage microbial reductions. The data are provided as machine-readable CSV files and an Excel workbook. Assays were reported as performed in triplicate. This deposit contains the processed numerical values reported in Tables 1–6 of the manuscript. Individual replicate-level laboratory observations and original statistical-analysis files are not included in this version. Where available, the original raw experimental records should be added to the repository deposit.

Steps to reproduce

Extraction: Dried Uziza (P. guineense), Uda (X. aethiopica), Ehuru (M. myristica), and Aidan fruit (T. tetraptera) were sourced from Anyigba, Kogi State, Nigeria, cleaned, air-dried, and pulverised. Extraction was by cold maceration: 50 g powder soaked in 500 mL of 70% ethanol, 72 h at room temperature with intermittent agitation. Filtrate (Whatman No. 1) was concentrated by rotary evaporation to a 100% (w/v) stock; 50% and 25% dilutions were prepared in sterile distilled water. Ten blends (AB, AC, AD, BC, BD, ABC, ACD, BCD, ABCD) were made from equal-proportion mixtures of stock extracts; CD was not tested against E. coli. Organisms: E. coli and S. aureus (bacteria) and A. niger (fungus). Bacterial inoculum standardised to 0.5 McFarland (~1.5×10⁸ CFU/mL), diluted 1:100 to ~1.5×10⁶ CFU/mL. A. niger spores from 5-7-day cultures on Sabouraud Dextrose Agar, harvested in saline with 0.1% Tween 80, adjusted to ~1×10⁶ spores/mL. Susceptibility assays (Tables 1, 2, 4, 5): Agar well diffusion on Mueller-Hinton agar (bacteria) and Sabouraud Dextrose Agar (fungus), 100%/50%/25% concentrations, standard antimicrobial as positive control, solvent as negative control. Bacterial plates: 37°C, 24 h. Fungal plates: 25-28°C, 48-72 h. Zones read in mm, triplicate (n=3), mean ± SD. MIC/MBC/MFC (Table 3): Broth microdilution, two-fold serial dilutions, same inoculum and incubation conditions as above. Produce trial (Table 6): Single P. guineense extract and ABCD blend (best/weakest in vitro) tested against a commercial sanitizer and water wash. Cucumber and tomato (two lots each) fully immersed in 500 mL treatment solution, 10 min contact, air-dried aseptically. TVBC by serial dilution and plate count on nutrient agar (CFU/g) before and after treatment; % reduction calculated vs. pre-treatment count. Statistics: IBM SPSS Statistics; one-way ANOVA with Tukey's HSD post-hoc; means with different superscript letters within a row/column differ at p < 0.05.

Institutions

• Kogi State University

  Kogi State, Dekina

Categories

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