seven physiological indicators

Description

This study was conducted based on the hypothesis that Rosa roxburghii employs stress-specific antioxidant strategies rather than a universal defense response under different abiotic stresses. To test this hypothesis, leaves were subjected to four representative abiotic stresses, including drought, salinity, ultraviolet (UV) radiation, and temperature stress. The datasets include measurements of reactive oxygen species (ROS) accumulation, antioxidant enzyme activities [superoxide dismutase (SOD) and peroxidase (POD)], and non-enzymatic antioxidant compounds, including flavonoids, vitamin C (Vc), and free amino acids (FAA). All physiological and biochemical parameters were determined using independent biological replicates and expressed as mean ± SE. Statistical differences were evaluated using one-way ANOVA followed by Tukey’s multiple comparison test (P < 0.05), and correlation analysis was performed to evaluate the relationships among ROS production and antioxidant components. The collected data revealed distinct antioxidant response patterns among different abiotic stresses. UV stress strongly stimulated oxidative responses by increasing superoxide anion (O₂⁻·) production and activating SOD activity, accompanied by enhanced accumulation of Vc and FAA. These results indicate that UV exposure rapidly activates both enzymatic and metabolic antioxidant defenses to alleviate oxidative pressure. In contrast, drought and salinity stresses exhibited time-dependent antioxidant regulation, characterized by rapid activation of antioxidant enzymes during early stress stages followed by reduced activity under prolonged stress conditions. Severe drought and salt stress also promoted FAA accumulation, suggesting a potential role of amino acids in stress adaptation. Temperature stress showed limited effects on SOD and POD activities but significantly influenced flavonoid accumulation, indicating that temperature conditions primarily regulate antioxidant capacity through metabolic antioxidants. Furthermore, UV-treated callus tissues displayed a distinct antioxidant response compared with leaf tissues. Callus showed significant flavonoid accumulation without obvious changes in SOD activity or Vc content, suggesting tissue-specific regulation of antioxidant metabolism. These datasets demonstrate that Rosa roxburghii coordinates enzymatic and non-enzymatic antioxidants in a stress-dependent and tissue-specific manner to maintain ROS homeostasis. The data provide valuable physiological evidence for understanding antioxidant adaptation mechanisms and can serve as a foundation for future molecular studies investigating stress-responsive regulatory networks.

Institutions

• Guizhou Minzu University

  Guizhou, Guiyang

Categories

Funders

• Guizhou Provincial Science and Technology Department

  Guizhou, Guiyang

Licence