Santiago et al., 2026. “Functional Senescence-Associated miRNAs Disrupt lncRNA-KCNQ1OT1-Mediated Heterochromatin Silencing to Drive Senescence”
Description
Santiago et al., 2026. “Functional Senescence-Associated miRNAs Disrupt lncRNA-KCNQ1OT1-Mediated Heterochromatin Silencing to Drive Senescence” Raw miRNA sensor reads (forward and reverse) segregated by barcode tag. The sensor library is as referenced in Mullokandov et al. (2012). Barcode Key: Timepoint Sort Bin Sequence Total Reads 0 0--2 AACC 16020584 0 0--3 AAGG 16391203 0 0--4 CACT 15694946 4 4--2 CCGG 15790207 4 4--3 GGAA 15296033 4 4--4 GGCC 15208110 10 10--2 TTAA 14599477 10 10--3 TTCC 15632574 10 10--4 AATT 13613088 Files: Collapsed_AACC.txt Collapsed_AACC_.txt Collapsed_AAGG.txt Collapsed_AAGG_.txt Collapsed_AATT.txt Collapsed_AATT_.txt Collapsed_CACT.txt Collapsed_CACT_.txt Collapsed_CCGG.txt Collapsed_CCGG_.txt Collapsed_GGAA.txt Collapsed_GGAA_.txt Collapsed_GGCC.txt Collapsed_GGCC_.txt Collapsed_TTAA.txt Collapsed_TTAA_.txt Collapsed_TTCC.txt Collapsed_TTCC_.txt Mullokandov G, Baccarini A, Ruzo A, Jayaprakash AD, Tung N, Israelow B, Evans MJ, Sachidanandam R, Brown BD. High-throughput assessment of microRNA activity and function using microRNA sensor and decoy libraries. Nat Methods. 2012 Jul 1;9(8):840-6. doi: 10.1038/nmeth.2078.
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Steps to reproduce
The proportion of sensor reads in each bin (barcode) are a function of the frequency of each sensor relative to the total reads for each sensor for a particular timepoint (comprising three barcodes).