CLINICOPATHOLOGICAL STUDY OF ROLE OF CD 34 EXPRESSIONS IN THE STROMA OF PREMALIGNANT AND MALIGNANT LESIONS OF CERVIX
All 215 collected were grouped into two broad categories, premalignant and malignant cervical lesions. A total of 72 (33.5%) cases of premalignant lesions and 143 (66.5%) cases of malignant lesions were studied under various headings or parameters,as given in master chart. for e.g 1. age group 2. presenting symptoms 3. risk factors 4. histopathological diagnosis 5. immunohistochemical analysis
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MATERIAL AND METHODS: The present study was conducted in the Department of Pathology, JNMCH, Aligarh (U.P) from July 2014 to July 2016. A total of two hundred fifteen (215) patients suffering from premalignant and malignant lesions of cervix were evaluated by H&E–stained tissue sections. Immunohistochemistry was performed using the standard avidin biotin complex (ABC)-peroxidase method and 3,3′-diaminobenzidine (DAB) as chromogen. CD34 antigen was detected by means of a monoclonal antibody (QBEND10, Dako, Hamburg, Germany, dilution 1:50) after microwave pretreatment.