A target expression threshold dictates invader defense and autoimmunity by CRISPR-Cas13

Published: 9 June 2022| Version 1 | DOI: 10.17632/97s4hpvztb.1
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Description

Unprocessed gel images Fig. S1B and S1D. Fig. S1B - Impact of inducing target expression on total RNA. From left to right: Biological replicate 1 (T1 uninduced, T1 induced, T2 uninduced, T2 induced, NT uninduced, NT induced), Biological replicate 2 (same order). Fig. S1D - Lack of total RNA degradation following targeting of chromosomally-encoded transcripts under the expression threshold. From left to right: Biological replicate 1 (lrhA Cas13a, soxS Cas13a, rdgC Cas13a, NT Cas13a, lrhA cBAD33, soxS cBAD33, rdgC cBAD33, NT cBAD33), Biological replicate 2 (same order) and 3 (same order).

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Institutions

Helmholtz-Institut fur RNA-basierte Infektionsforschung

Categories

RNA, CRISPR

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