Part1_Petromurin C induces protective autophagy and apoptosis in FLT3-ITD-positive AML: synergy with gilteritinib

Published: 13 Dec 2019 | Version 1 | DOI: 10.17632/9dhbprzxh2.1
Contributor(s):

Description of this data

Treatment of acute myeloid leukemia (AML) remains inefficient due to drug resistance and relapse, particularly in patients with FMS-like tyrosine kinase (FLT)3-internal tandem duplication (ITD). Marine-derived natural products have recently been used for drug development against AML. We show here that petromurin C, isolated from the culture extract of the marine-derived fungus Aspergillus candidus KUFA0062, which was isolated from the marine sponge Epipolasis sp., induces early autophagy followed by apoptotic cell death via activation of the intrinsic cell death pathway concomitant with mitochondrial stress and downregulation of Mcl-1 in FLT3-ITD mutated MV4-11 cells. Moreover, petromurin C synergized with the clinically-used FLT3 inhibitor gilteritinib at sub-toxic concentrations. Altogether our results suggest that petromurin C could act as an anti-leukemic agent alone or in combination with gilteritinib.

Experiment data files

  • Figure 1
    Cite
    • Figure 1
      Cite
  • Figure 2
    Cite
    • A_Trypan Blue Assay in MV4-11
      Cite
    • B_Trypan Blue Assay in U937
      Cite
    • C_Colony Formation Assay in MV4-11
      Cite
      • 1st
        Cite
      • 2nd
        Cite
      • 3 sets combined
        Cite
      • 3rd
        Cite
      • Representatives
        Cite
    • D_Colony Formation Assay in U937
      Cite
      • 1st
        Cite
      • 2nd
        Cite
      • 3 sets combined
        Cite
      • 3rd
        Cite
      • Representatives
        Cite
    • E_zebrafish toxicity
      Cite
      • fish pictures_24h
        Cite
        • 0uM
          Cite
          • fish_0uM_10_24h
            Cite
          • fish_0uM_11_24h
            Cite
          • fish_0uM_12_24h
            Cite
          • fish_0uM_13_24h
            Cite
          • fish_0uM_14_24h
            Cite
          • fish_0uM_15_24h
            Cite
          • fish_0uM_1_24h
            Cite
          • fish_0uM_2_24h
            Cite
          • fish_0uM_3_24h
            Cite
          • fish_0uM_4_24h
            Cite
          • fish_0uM_5_24h
            Cite
          • fish_0uM_6_24h
            Cite
          • fish_0uM_7_24h
            Cite
          • fish_0uM_8_24h
            Cite
          • fish_0uM_9_24h
            Cite
        • 10uM
          Cite
          • fish_10uM_10_24h
            Cite
          • fish_10uM_11_24h
            Cite
          • fish_10uM_12_24h
            Cite
          • fish_10uM_13_24h
            Cite
          • fish_10uM_14_24h
            Cite
          • fish_10uM_15_24h
            Cite
          • fish_10uM_1_24h
            Cite
          • fish_10uM_2_24h
            Cite
          • fish_10uM_3_24h
            Cite
          • fish_10uM_4_24h
            Cite
          • fish_10uM_5_24h
            Cite
          • fish_10uM_6_24h
            Cite
          • fish_10uM_7_24h
            Cite
          • fish_10uM_8_24h
            Cite
          • fish_10uM_9_24h
            Cite
        • 1uM
          Cite
          • fish_1uM_10_24h
            Cite
          • fish_1uM_11_24h
            Cite
          • fish_1uM_12_24h
            Cite
          • fish_1uM_13_24h
            Cite
          • fish_1uM_14_24h
            Cite
          • fish_1uM_15_24h
            Cite
          • fish_1uM_1_24h
            Cite
          • fish_1uM_2_24h
            Cite
          • fish_1uM_3_24h
            Cite
          • fish_1uM_4_24h
            Cite
          • fish_1uM_5_24h
            Cite
          • fish_1uM_6_24h
            Cite
          • fish_1uM_7_24h
            Cite
          • fish_1uM_8_24h
            Cite
          • fish_1uM_9_24h
            Cite
        • 30uM
          Cite
          • fish_30uM_10_24h
            Cite
          • fish_30uM_11_24h
            Cite
          • fish_30uM_12_24h
            Cite
          • fish_30uM_13_24h
            Cite
          • fish_30uM_14_24h
            Cite
          • fish_30uM_15_24h
            Cite
          • fish_30uM_1_24h
            Cite
          • fish_30uM_2_24h
            Cite
          • fish_30uM_3_24h
            Cite
          • fish_30uM_4_24h
            Cite
          • fish_30uM_5_24h
            Cite
          • fish_30uM_6_24h
            Cite
          • fish_30uM_7_24h
            Cite
          • fish_30uM_8_24h
            Cite
          • fish_30uM_9_24h
            Cite
        • 50uM
          Cite
          • fish_50uM_10_24h
            Cite
          • fish_50uM_11_24h
            Cite
          • fish_50uM_12_24h
            Cite
          • fish_50uM_13_24h
            Cite
          • fish_50uM_14_24h
            Cite
          • fish_50uM_15_24h
            Cite
          • fish_50uM_1_24h
            Cite
          • fish_50uM_2_24h
            Cite
          • fish_50uM_3_24h
            Cite
          • fish_50uM_4_24h
            Cite
          • fish_50uM_5_24h
            Cite
          • fish_50uM_6_24h
            Cite
          • fish_50uM_7_24h
            Cite
          • fish_50uM_8_24h
            Cite
          • fish_50uM_9_24h
            Cite
        • 5uM
          Cite
          • fish_5uM_10_24h
            Cite
          • fish_5uM_11_24h
            Cite
          • fish_5uM_12_24h
            Cite
          • fish_5uM_13_24h
            Cite
          • fish_5uM_14_24h
            Cite
          • fish_5uM_15_24h
            Cite
          • fish_5uM_1_24h
            Cite
          • fish_5uM_2_24h
            Cite
          • fish_5uM_3_24h
            Cite
          • fish_5uM_4_24h
            Cite
          • fish_5uM_5_24h
            Cite
          • fish_5uM_6_24h
            Cite
          • fish_5uM_7_24h
            Cite
          • fish_5uM_8_24h
            Cite
          • fish_5uM_9_24h
            Cite
      • fish pictures_48h
        Cite
        • fish 0uM_48h
          Cite
          • fish_0uM_10_48h
            Cite
          • fish_0uM_11_48h
            Cite
          • fish_0uM_12_48h
            Cite
          • fish_0uM_13_48h
            Cite
          • fish_0uM_14_48h
            Cite
          • fish_0uM_15_48h
            Cite
          • fish_0uM_1_48h
            Cite
          • fish_0uM_2_48h
            Cite
          • fish_0uM_3_48h
            Cite
          • fish_0uM_4_48h
            Cite
          • fish_0uM_5_48h
            Cite
          • fish_0uM_6_48h
            Cite
          • fish_0uM_7_48h
            Cite
          • fish_0uM_8_48h
            Cite
          • fish_0uM_9_48h
            Cite
        • fish 10uM_48h
          Cite
          • fish_10uM_10_48h
            Cite
          • fish_10uM_11_48h
            Cite
          • fish_10uM_12_48h
            Cite
          • fish_10uM_13_48h
            Cite
          • fish_10uM_14_48h
            Cite
          • fish_10uM_15_48h
            Cite
          • fish_10uM_1_48h
            Cite
          • fish_10uM_2_48h
            Cite
          • fish_10uM_3_48h
            Cite
          • fish_10uM_4_48h
            Cite
          • fish_10uM_5_48h
            Cite
          • fish_10uM_6_48h
            Cite
          • fish_10uM_7_48h
            Cite
          • fish_10uM_8_48h
            Cite
          • fish_10uM_9_48h
            Cite
        • fish 1uM_48h
          Cite
          • fish_1uM_10_48h
            Cite
          • fish_1uM_11_48h
            Cite
          • fish_1uM_12_48h
            Cite
          • fish_1uM_13_48h
            Cite
          • fish_1uM_14_48h
            Cite
          • fish_1uM_15_48h
            Cite
          • fish_1uM_1_48h
            Cite
          • fish_1uM_2_48h
            Cite
          • fish_1uM_3_48h
            Cite
          • fish_1uM_4_48h
            Cite
          • fish_1uM_5_48h
            Cite
          • fish_1uM_6_48h
            Cite
          • fish_1uM_7_48h
            Cite
          • fish_1uM_8_48h
            Cite
          • fish_1uM_9_48h
            Cite
        • fish 30uM_48h
          Cite
          • fish_30uM_10_48h
            Cite
          • fish_30uM_11_48h
            Cite
          • fish_30uM_12_48h
            Cite
          • fish_30uM_13_48h
            Cite
          • fish_30uM_14_48h
            Cite
          • fish_30uM_15_48h
            Cite
          • fish_30uM_1_48h
            Cite
          • fish_30uM_2_48h
            Cite
          • fish_30uM_3_48h
            Cite
          • fish_30uM_4_48h
            Cite
          • fish_30uM_5_48h
            Cite
          • fish_30uM_6_48h
            Cite
          • fish_30uM_7_48h
            Cite
          • fish_30uM_8_48h
            Cite
          • fish_30uM_9_48h
            Cite
        • fish 50uM_48h
          Cite
          • fish_50uM_10_48h
            Cite
          • fish_50uM_11_48h
            Cite
          • fish_50uM_12_48h
            Cite
          • fish_50uM_13_48h
            Cite
          • fish_50uM_14_48h
            Cite
          • fish_50uM_15_48h
            Cite
          • fish_50uM_1_48h
            Cite
          • fish_50uM_2_48h
            Cite
          • fish_50uM_3_48h
            Cite
          • fish_50uM_4_48h
            Cite
          • fish_50uM_5_48h
            Cite
          • fish_50uM_6_48h
            Cite
          • fish_50uM_7_48h
            Cite
          • fish_50uM_8_48h
            Cite
          • fish_50uM_9_48h
            Cite
        • fish 5uM_48h
          Cite
          • fish_5uM_10_48h
            Cite
          • fish_5uM_11_48h
            Cite
          • fish_5uM_12_48h
            Cite
          • fish_5uM_13_48h
            Cite
          • fish_5uM_14_48h
            Cite
          • fish_5uM_15_48h
            Cite
          • fish_5uM_1_48h
            Cite
          • fish_5uM_2_48h
            Cite
          • fish_5uM_3_48h
            Cite
          • fish_5uM_4_48h
            Cite
          • fish_5uM_5_48h
            Cite
          • fish_5uM_6_48h
            Cite
          • fish_5uM_7_48h
            Cite
          • fish_5uM_8_48h
            Cite
          • fish_5uM_9_48h
            Cite
      • fish pictures_72h
        Cite
        • fish 0uM_72h
          Cite
          • fish_0uM_10_72h
            Cite
          • fish_0uM_11_72h
            Cite
          • fish_0uM_12_72h
            Cite
          • fish_0uM_13_72h
            Cite
          • fish_0uM_14_72h
            Cite
          • fish_0uM_15_72h
            Cite
          • fish_0uM_1_72h
            Cite
          • fish_0uM_2_72h
            Cite
          • fish_0uM_3_72h
            Cite
          • fish_0uM_4_72h
            Cite
          • fish_0uM_5_72h
            Cite
          • fish_0uM_6_72h
            Cite
          • fish_0uM_7_72h
            Cite
          • fish_0uM_8_72h
            Cite
          • fish_0uM_9_72h
            Cite
        • fish 10uM_72h
          Cite
          • fish_10uM_10_72h
            Cite
          • fish_10uM_11_72h
            Cite
          • fish_10uM_12_72h
            Cite
          • fish_10uM_13_72h
            Cite
          • fish_10uM_14_72h
            Cite
          • fish_10uM_15_72h
            Cite
          • fish_10uM_1_72h
            Cite
          • fish_10uM_2_72h
            Cite
          • fish_10uM_3_72h
            Cite
          • fish_10uM_4_72h
            Cite
          • fish_10uM_5_72h
            Cite
          • fish_10uM_6_72h
            Cite
          • fish_10uM_7_72h
            Cite
          • fish_10uM_8_72h
            Cite
          • fish_10uM_9_72h
            Cite
        • fish 1uM_72h
          Cite
          • fish_1uM_10_72h
            Cite
          • fish_1uM_11_72h
            Cite
          • fish_1uM_12_72h
            Cite
          • fish_1uM_13_72h
            Cite
          • fish_1uM_14_72h
            Cite
          • fish_1uM_15_72h
            Cite
          • fish_1uM_1_72h
            Cite
          • fish_1uM_2_72h
            Cite
          • fish_1uM_3_72h
            Cite
          • fish_1uM_4_72h
            Cite
          • fish_1uM_5_72h
            Cite
          • fish_1uM_6_72h
            Cite
          • fish_1uM_7_72h
            Cite
          • fish_1uM_8_72h
            Cite
          • fish_1uM_9_72h
            Cite
        • fish 30uM_72h
          Cite
          • fish_30uM_10_72h
            Cite
          • fish_30uM_11_72h
            Cite
          • fish_30uM_12_72h
            Cite
          • fish_30uM_13_72h
            Cite
          • fish_30uM_14_72h
            Cite
          • fish_30uM_15_72h
            Cite
          • fish_30uM_1_72h
            Cite
          • fish_30uM_2_72h
            Cite
          • fish_30uM_3_72h
            Cite
          • fish_30uM_4_72h
            Cite
          • fish_30uM_5_72h
            Cite
          • fish_30uM_6_72h
            Cite
          • fish_30uM_7_72h
            Cite
          • fish_30uM_8_72h
            Cite
          • fish_30uM_9_72h
            Cite
        • fish 50uM_72h
          Cite
          • fish_50uM_10_72h
            Cite
          • fish_50uM_11_72h
            Cite
          • fish_50uM_12_72h
            Cite
          • fish_50uM_13_72h
            Cite
          • fish_50uM_14_72h
            Cite
          • fish_50uM_15_72h
            Cite
          • fish_50uM_1_72h
            Cite
          • fish_50uM_2_72h
            Cite
          • fish_50uM_3_72h
            Cite
          • fish_50uM_4_72h
            Cite
          • fish_50uM_5_72h
            Cite
          • fish_50uM_6_72h
            Cite
          • fish_50uM_7_72h
            Cite
          • fish_50uM_8_72h
            Cite
          • fish_50uM_9_72h
            Cite
        • fish 5uM_72h
          Cite
          • fish_5uM_10_72h
            Cite
          • fish_5uM_11_72h
            Cite
          • fish_5uM_12_72h
            Cite
          • fish_5uM_13_72h
            Cite
          • fish_5uM_14_72h
            Cite
          • fish_5uM_15_72h
            Cite
          • fish_5uM_1_72h
            Cite
          • fish_5uM_2_72h
            Cite
          • fish_5uM_3_72h
            Cite
          • fish_5uM_4_72h
            Cite
          • fish_5uM_5_72h
            Cite
          • fish_5uM_6_72h
            Cite
          • fish_5uM_7_72h
            Cite
          • fish_5uM_8_72h
            Cite
          • fish_5uM_9_72h
            Cite
      • heart beat
        Cite
      • length
        Cite
      • Representative pictures
        Cite
      • viability
        Cite
    • Table 2_IC50 values
      Cite
  • Figure 3
    Cite
    • A_Hoechst and PI of MV4-11
      Cite
      • MV4-11_24h
        Cite
        • MV4-11 with and without z-vad_24h_1st
          Cite
          • MV4-11_24h_1st
            Cite
            • 0
              Cite
            • 1
              Cite
            • 10
              Cite
            • 30
              Cite
            • 5
              Cite
            • 50
              Cite
          • MV4-11_z-vad_24h_1st
            Cite
            • 0+zvad
              Cite
            • 1+zvad
              Cite
            • 10+zvad
              Cite
            • 30+zvad
              Cite
            • 5+zvad
              Cite
            • 50+zvad
              Cite
        • MV4-11 with and without z-vad_24h_2nd
          Cite
          • MV4-11_24h_2nd
            Cite
            • 0
              Cite
            • 1
              Cite
            • 10
              Cite
            • 30
              Cite
            • 5
              Cite
            • 50
              Cite
          • MV4-11_zvad_24h_2nd
            Cite
            • 0+zvad_2nd
              Cite
            • 1+zvad_2nd
              Cite
            • 10+zvad_2nd
              Cite
            • 30+zvad_2nd
              Cite
            • 5+zvad_2nd
              Cite
            • 50+zvad_2nd
              Cite
        • MV4-11 with and without z-vad_24h_3rd
          Cite
          • MV4-11_24h_3rd
            Cite
            • 0_3rd
              Cite
            • 10_3rd
              Cite
            • 1_3rd
              Cite
            • 30_3rd
              Cite
            • 50_3rd
              Cite
            • 5_3rd
              Cite
          • MV4-11_zvad_24h_3rd
            Cite
            • 0+zvad_3rd
              Cite
            • 1+zvad_3rd
              Cite
            • 10+zvad_3rd
              Cite
            • 30+zvad_3rd
              Cite
            • 5+zvad_3rd
              Cite
            • 50+zvad_3rd
              Cite
      • MV4-11_48h
        Cite
        • MV4-11 with and without z-vad_48h_1st
          Cite
          • MV4-11_48h_1st
            Cite
            • 0
              Cite
            • 1
              Cite
            • 10
              Cite
            • 30
              Cite
            • 5
              Cite
            • 50
              Cite
          • MV4-11_z-vad_48h_1st
            Cite
            • 0+zvad_1st
              Cite
            • 1+zvad_1st
              Cite
            • 10+zvad_1st
              Cite
            • 30+zvad_1st
              Cite
            • 5+zvad_1st
              Cite
            • 50+zvad_1st
              Cite
        • MV4-11 with and without z-vad_48h_2nd
          Cite
          • MV4-11_48h_2nd
            Cite
            • 0_2nd
              Cite
            • 10_2nd
              Cite
            • 1_2nd
              Cite
            • 30_2nd
              Cite
            • 50_2nd
              Cite
            • 5_2nd
              Cite
          • MV4-11_z-vad_48h_2nd
            Cite
            • 0+zvad_2nd
              Cite
            • 1+zvad_2nd
              Cite
            • 10+zvad_2nd
              Cite
            • 30+zvad_2nd
              Cite
            • 5+zvad_2nd
              Cite
            • 50+zvad_2nd
              Cite
        • MV4-11 with and without z-vad_48h_3rd
          Cite
          • MV4-11_48h_3rd
            Cite
            • 0_48h
              Cite
            • 10_48h
              Cite
            • 1_48h
              Cite
            • 30_48h
              Cite
            • 50_48h
              Cite
            • 5_48h
              Cite
          • MV4-11_z-vad_48h_3rd
            Cite
            • 0+zvad_3rd
              Cite
            • 1+zvad_3rd
              Cite
            • 10+zvad_3rd
              Cite
            • 30+zvad_3rd
              Cite
            • 5+zvad_3rd
              Cite
            • 50+zvad_3rd
              Cite
      • MV4-11_72h
        Cite
        • MV4-11 with and without z-vad_72h_1st
          Cite
          • MV4-11_72h_1st
            Cite
            • 0_72h
              Cite
            • 10_72h
              Cite
            • 1_72h
              Cite
            • 30_72h
              Cite
            • 50_72h
              Cite
            • 5_72h
              Cite
          • MV4-11_z-vad_72h_1st
            Cite
            • 0+zvad_72h
              Cite
            • 1+zvad_72h
              Cite
            • 10+zvad_72h
              Cite
            • 30+zvad_72h
              Cite
            • 5+zvad_72h
              Cite
            • 50+zvad_72h
              Cite
        • MV4-11 with and without z-vad_72h_2nd
          Cite
          • MV4-11_72h_2nd
            Cite
            • 0_72h
              Cite
            • 10_72h
              Cite
            • 1_72h
              Cite
            • 30_72h
              Cite
            • 50_72h
              Cite
            • 5_72h
              Cite
          • MV4-11_z-vad_72h_2nd
            Cite
            • 0+zvad_72h
              Cite
            • 1+zvad_72h
              Cite
            • 10+zvad_72h
              Cite
            • 30+zvad_72h
              Cite
            • 5+zvad_72h
              Cite
            • 50+zvad_72h
              Cite
        • MV4-11 with and without z-vad_72h_3rd
          Cite
          • MV4-11_72h_3rd
            Cite
            • 0_72h
              Cite
            • 10_72h
              Cite
            • 1_72h
              Cite
            • 30_72h
              Cite
            • 50_72h
              Cite
            • 5_72h
              Cite
          • MV4-11_z-vad_72h_3rd
            Cite
            • 0+zvad_72h
              Cite
            • 1+zvad_72h
              Cite
            • 10+zvad_72h
              Cite
            • 30+zvad_72h
              Cite
            • 5+zvad_72h
              Cite
            • 50+zvad_72h
              Cite
      • MV4-11_eto_4h
        Cite
        • eto+zvad_4h
          Cite
          • 1st
            Cite
          • 2nd
            Cite
          • 3rd
            Cite
        • eto_4h
          Cite
          • 1st
            Cite
          • 2nd
            Cite

Latest version

  • Version 1

    2019-12-13

    Published: 2019-12-13

    DOI: 10.17632/9dhbprzxh2.1

    Cite this dataset

    Ha, You Na (2019), “Part1_Petromurin C induces protective autophagy and apoptosis in FLT3-ITD-positive AML: synergy with gilteritinib”, Mendeley Data, v1 http://dx.doi.org/10.17632/9dhbprzxh2.1

Statistics

Views: 4261
Downloads: 680

Categories

Health Sciences

Licence

CC BY 4.0 Learn more

The files associated with this dataset are licensed under a Creative Commons Attribution 4.0 International licence.

What does this mean?
You can share, copy and modify this dataset so long as you give appropriate credit, provide a link to the CC BY license, and indicate if changes were made, but you may not do so in a way that suggests the rights holder has endorsed you or your use of the dataset. Note that further permission may be required for any content within the dataset that is identified as belonging to a third party.

Report