African Yam Been drought tolerance studies dataset
Description
This data set contains data generated from studies in nine (9) selected accessions of African Yam Bean [Sphenostylis stenocarpa (Hochst. ex A. Rich.) Harms] conducted at the University of Calabar, Nigeria. The set contains data of physiology and stress-antioxidant studies. Drought stress response index from the physiology and antioxidant data was also calculated and added to the dataset.
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Steps to reproduce
The experiment was arranged as a 9 x 3 factorial in a completely randomized design (CRD), consisting of nine successfully germinated AYB accessions and three watering regimes. Each accession-treatment combination was replicated three times, giving a total of 81 experimental pots. The pot was regarded as the experimental unit. The three watering regimes were designated as control, moderate drought stress, and severe drought stress. Seeds of each accession were sown in perforated polyethylene planting bags filled with 3 kg of soil. No fertilizer was applied to avoid nutrient-induced variation in stress response. Ten seeds were initially sown per pot. At 14 days after sowing (DAS), shortly after seedling establishment, the seedlings were thinned to five uniform plants per pot. During the establishment phase, all pots received 250 mL of water at two-day intervals for the first 14 DAS. Differential watering commenced after the establishment phase to induce progressive drought stress. Control pots continued to receive 250 mL of water every two days, while moderate and severe drought-stress treatments received 150 mL and 100 mL of water every two days, respectively. Physiological and biochemical observations were taken at 14, 21, and 28 days after the commencement of drought-stress treatment. These sampling points were selected to capture changes in plant response as drought exposure progressed from early to more advanced stress stages.
Institutions
- University of CalabarCross River State, Calabar