Effects of Cannabivarin and Tetrahydrocannabivarin in nociception behavior in Caenorhabditis elegans
Description
This data contains the proteomics results when studying the effect of two compounds, cannabivarin and tetrahydrocannabivarin, in C. elegans,
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The experiment was carried out on a Thermo Scientific Vanquish Neo UHPLC system in trap-and-elute mode. The configuration included a Thermo Scientific PepMap Neo 5 μm C18 300 μm × 5 mm trap cartridge and a Thermo Scientific PepMap Neo C18 2 μm × 75 μm × 500 mm nano column. Two micrograms of digested proteins were loaded onto the trap column and desalted with solvent A [0.1% formic acid in water] at a 20 μL/min flow rate for 0.5 minutes. Peptide elution was carried out using a linear gradient of 5% to 35% solvent B [80% acetonitrile, 20% water, 0.1% formic acid] over 135 minutes, followed by a 2-step column wash at 50% solvent B for 10 minutes and 80% for 10 minutes. The flow rate was maintained at 200 nL/min. Afterwards, the column was equilibrated to the initial solvent composition (5% solvent B) by running 10 column volumes. Data acquisition was conducted on a Thermo Scientific Q Exactive Plus Orbitrap Mass Spectrometer (San Jose, CA, USA) equipped with a Nanospray Flex ion source. Data collection was performed in positive ion mode with a nanospray voltage of 2.2 kV, and the ion transfer tube was maintained at 220°C. The mass spectrometer was operated in data-dependent acquisition (DDA) mode, utilizing a TOP-12 acquisition strategy. A high-resolution MS1 survey scan (m/z 375– 1200) was acquired at a resolution of 70,000 (FWHM), with an automatic gain control (AGC) target value of 1 × 10 and a maximum injection time of 100 ms. The 12 most intense precursor ions exceeding an intensity threshold of 1 × 10 were selected for fragmentation via higher-energy collisional dissociation (HCD) at a normalized collision energy (NCE) of 27. MS2 spectra were acquired at a resolution of 17,500 (FWHM) using an AGC target value of 2 × 10 and a maximum injection time of 100 ms.
Institutions
- Universite de Montreal Faculte de medecine veterinaire