Clonal amplification and weak genetic structuring in the rumen fluke Calicophoron daubneyi at a regional scale in the French Pyrenees
Description
Complete dataset.xlsx Genotypic dataset for 187 Calicophoron daubneyi individuals collected from seven localities in the eastern French Pyrenees (January–February 2019) and genotyped at 10 microsatellite loci (Cal.daub.04, 08, 10, 13, 18, 19, 21, 22, 23, 25). Each row corresponds to one individual trematode. Columns include: sampling locality code (pop), individual identifier (ind), geographic coordinates (Lat/Long, decimal degrees), collection date, host identifier (one bovine host per locality), unique individual code (SiteIndiv), genotypes expressed as concatenated allele sizes in base pairs (e.g., 236236 for a homozygote; 236284 for a heterozygote; 000000 for missing data), allele sizes in separate columns (Loc04 to Loc25), and multilocus genotype (MLG) assignment based on a Neighbor-Joining tree (unique genotypes labelled "SingularXX"; shared genotypes labelled "MLGX"). Clone-corrected dataset.xlsx Clone-corrected genotypic dataset for Calicophoron daubneyi, retaining a single representative per unique multilocus genotype (MLG). This dataset comprises 130 unique MLGs identified among the 187 genotyped individuals from seven localities in the eastern French Pyrenees. For each MLG, the most complete and most heterozygous genotype was retained. Column structure is identical to the complete dataset. geosphere.R R script using the geosphere package to compute pairwise geographic distances between the seven Calicophoron daubneyi sampling localities based on decimal GPS coordinates.