ZNF574 is a Quality Control Factor for Defective Ribosome Biogenesis Intermediates Akers et al

Published: 10 March 2025| Version 1 | DOI: 10.17632/9mxxrftwgv.1
Contributor:
Kamena Kostova

Description

U2OS cells CRISPRi expressing uL16 wild type or mutant together with sgRNAs targeting Gal4 (control) or ZNF574 (20210203_rep1, 2, 3) HEK293T cells stained with antibody raised against ZNF574 (ZNF574_Ab) PLA assays detecting interactions between FLAG-tagged wild type or mutant uL16 and endogenous ZNF574 in HEK293T cells (20241213_PLA) Zebrafish images

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Steps to reproduce

A detailed description of the methods and data analysis is provided in the STAR Methods section of the manuscript.

Institutions

  • Stowers Institute for Medical Research
  • Carnegie Institution for Science

Categories

Cell Biology

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