ZNF574 is a Quality Control Factor for Defective Ribosome Biogenesis Intermediates Akers et al

Published: 10 March 2025| Version 1 | DOI: 10.17632/9mxxrftwgv.1
Contributor:
Kamena Kostova

Description

U2OS cells CRISPRi expressing uL16 wild type or mutant together with sgRNAs targeting Gal4 (control) or ZNF574 (20210203_rep1, 2, 3) HEK293T cells stained with antibody raised against ZNF574 (ZNF574_Ab) PLA assays detecting interactions between FLAG-tagged wild type or mutant uL16 and endogenous ZNF574 in HEK293T cells (20241213_PLA) Zebrafish images

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Steps to reproduce

A detailed description of the methods and data analysis is provided in the STAR Methods section of the manuscript.

Institutions

Stowers Institute for Medical Research, Carnegie Institution for Science

Categories

Cell Biology

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