JEB TRID treatment 2021
To determine the effect on aminoglycosides on keratinocytes from patients with junctional epidermolysis bullosa a hypothesis free gene expression study was performed. A keratinocyte cell line with a specific nonsense mutation in COL17A1 was treated with gentamicin or paromomycin or remained untreated. RNA was extracted in a triplicate experimental setting and submitted to RNA sequencing.
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RNA extracted from cells was sequenced by Illumina technology by Novogene. At Novogene, the state-of-the-art Illumina NovaSeq platforms are used for a paired-end 150 bp sequencing strategy (short-reads) to sequence the cDNA libraries. With strong sequencing capacity and rich experience, Novogene offers multiple services to meet various needs, including the access to not only eukaryotic mRNA sequencing (mRNA-seq) services, but also the studies on prokaryotic transcripts, non-coding RNAs, full-length isoforms (long-reads), whole transcriptomes, and meta-transcriptomes. (https://en.novogene.com/services/research-services/transcriptome-sequencing/mrna-sequencing-mrna-seq/). Sample Names: JC-NT1-3 not treated samples, JC-Gta1-3 treated with gentamicin, JC-Paro1-3 treated with paromycin.