Next generation sequencing analysis of gastric cancer
Gastric carcinoma tissues were obtained from 31 patients treated by surgical resection. The patients were subdivided into two groups according to the presence of peritoneal involvement, either macroscopically (P+) or microscopically (Cy+) at the time of the surgery: 19 patients had no peritoneal involvement at the time of diagnosis (P0 and Cy0) while 12 had peritoneal involvement, (P+ or Cy+). High-quality RNA was extracted from tumor gastric mucosa and healthy mucosa. Sequencing was performed on an Ion S5™ Sequencer with Torrent Suite™ Software v6 (Thermo Fisher Scientific). The analyses were performed with a range of fold <−2 and >+2 and a p value < 0.05, using Transcriptome Analysis Console Software (version 4.0.2), certified for AmpliSeq analysis.