Unlocking the Anisotropic Diffusion Puzzle in Crowded Biological Mimics: A Biophysical Revisit of Micro-Rheology with Soft vs. Hard Colloids
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The data for "Unlocking the Anisotropic Diffusion Puzzle in Crowded Biological Mimics: A Biophysical Revisit of Micro-Rheology with Soft vs. Hard Colloids". The data are obtained experimentally, via rheology, SAXS/SANS, DLS/DDLS.
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DLS and DDLS The experimental scattering angles are 60, 70, 80, 90, 100, 110, 120 and 130, and the corresponding range of q is 0.0126-0.0229 nm^(-1). The largest q×L ratio of the NRs (TMV and q=0.0229 nm^(-1)) used is 6.87 which is smaller than 8 and DDLS can observe the rotation without the high-order terms. The samples are loaded into glass tubes with diameter of 9 mm. The pseudo-cross-correlation set-up with double detectors is used for measurement, which can suppress the afterpulse effect of the photodetector and increase the temporal resolution to 10 ns59. The measurements are repeated three times for each sample. SAXS The SAXS experiment were conducted at BL10U1 beamline at Shanghai Synchrotron Radiation Facility. The wavelength of X-ray is 1.239 Å, the sample-to-detector distance is 5130 mm and the detector used is Pilatus-2M. The sample is injected into quartz capillary with optical distance of 1.5 mm for SAXS measurement at 298 K, the exposure time is 10 s. RAW is used to transform 2D image into 1D profile. SANS The SANS experiments were conducted at SANS beamline of China Spallation Neutron Source. Samples were loaded in Hellma quartz banjo cells with a 1 mm path length. The sample-to-detector distance was set to 5 m, employing an incident neutron wavelength range of 1.1 Å to 9.18 Å, corresponding to a q-range of 0.0044 Å⁻¹ to 0.7 Å⁻¹. Scattering patterns for each sample, along with those for the pure solvent, empty cell and empty beam, were collected in scattering and transmission modes respectively. Data reduction was performed using the software sansRZ, which included corrections for detector efficiency, incident beam intensity, sample transmission and thickness, as well as instrumental and empty cell backgrounds. The obtained 1D scattering profiles were placed on an absolute scale using a secondary calibration standard provided by the beamline. The solvent contribution was subtracted from the sample data according to the solvent volume fraction, whereas the incoherent background from the solute was treated as a flat background and accounted for by the background parameter of the fitting model. Rheology Rheological investigations were performed at Anton Paar MCR302 rheometer using a cone-plate rotor CP60, with a gap of 0.1 mm. The range of shear rate for hard sphere is 1-50 s^(-1) while the range for soft sphere is 1-100 s^(-1). η(ϕ) used for further analysis is obtained from the viscosity at zero-shear rate, and the regions for the extraction of η(ϕ) are (>20 s^(-1) and >50 s^(-1)) for hard sphere and soft sphere respectively, to avoid the effects of fluctuations at low shear rate.
Institutions
- South China University of TechnologyGuangdong, Guangzhou