Data for: Extinction darting predicts fear renewal in male but not female rats - behavioral and basolateral amygdala RNA-sequencing data
Description
Behavioral and transcriptomic data supporting the article "Extinction darting predicts fear renewal in male but not female rats: a behavioral dissociation with exploratory transcriptional correlates in the basolateral amygdala." Adult Sprague-Dawley rats (n = 18 per sex) underwent fear conditioning, three days of extinction in a large open-field arena permitting both passive (freezing) and active (darting) defensive responses, and a renewal test in a distinct context. Three hours after renewal, basolateral amygdala tissue from a subset of animals stratified within sex as high- or low-darters was processed for bulk RNA sequencing (12 libraries, 3 per subgroup). CONTENTS 01 - Gene-level expression matrix for all 12 libraries (19,007 genes; raw counts, FPKM, TPM, fold changes, raw and Benjamini-Hochberg adjusted p-values for both within-sex contrasts). 02 - Sample metadata (group, sex, darter status, contrast, total mapped reads). 03 - Male differentially expressed genes (MHD vs MLD; 98 candidates). 04 - Female differentially expressed genes (FHD vs FLD; 365 candidates). 05 - Per-animal behavioral data for the full cohort (36 animals): conditioning, early-extinction and renewal freezing, and extinction darting. README.txt - Column definitions and methods summary. The behavioral file reproduces every behavioral result reported in the article, including the male darting-renewal freezing association (r = -0.669, p = 0.002), the absence of a detectable association in females (r = 0.136, p = 0.591), and the darting x sex interaction on renewal freezing (p = 0.012). The transcriptomic analyses are exploratory: n = 3 per group with nominal, uncorrected thresholds, and no gene reaches an adjusted p < 0.05 in either sex. Raw sequence read files (FASTQ) are not available for this archival dataset; the gene-level count matrix is the earliest-stage sequencing data that remains.
Files
Steps to reproduce
BEHAVIOUR (05_behavioral_data_per_animal.csv) Adult Sprague-Dawley rats (n = 18 per sex) were habituated to a large open-field arena, given auditory fear conditioning, three days of extinction in the open field, and a renewal test in a distinct context. Freezing was scored automatically with ANY-maze. Darting was defined as movement exceeding 23.5 cm/s during the CS and was quantified during extinction only. Darter phenotype was assigned by a sex-specific median split of total extinction darts (male median = 11, female median = 9.5). Main results reproducible from this file: - Total extinction darting, males vs females: t(34) = 0.63, p = 0.533 - Male darting to renewal freezing: r = -0.669, p = 0.002 - Female darting to renewal freezing: r = 0.136, p = 0.591 - Darting x sex interaction (DV = renewal freezing): B = -0.039, p = 0.012 - Fisher r-to-z comparison of the two correlations: z = -2.59, p = 0.010 - Early-extinction freezing, males vs females: t(34) = 2.74, p = 0.010 - Renewal freezing, males vs females: t(34) = 2.21, p = 0.034 RNA SEQUENCING (01_gene_expression_matrix_12samples.csv, 02_sample_metadata.csv) Three hours after the renewal test, bilateral basolateral amygdala punches were taken from 3 animals per phenotype x sex group (12 libraries) and processed for bulk RNA sequencing (Macrogen; TruSeq Stranded mRNA; paired-end 101 bp; Illumina NovaSeq 6000; 42-60 million reads per library). Reads were aligned to rn6 with HISAT2 and differential expression was assessed with DESeq2 separately within each sex. DIFFERENTIAL EXPRESSION (03_, 04_) Candidate gene lists were filtered at nominal thresholds (absolute fold change > 1.5 and raw p < 0.05). These analyses are exploratory: n = 3 per group, thresholds are uncorrected, and no gene reaches a Benjamini-Hochberg adjusted p < 0.05 in either sex. Raw sequence read files (FASTQ) are not available for this archival dataset; the gene-level count matrix in file 01 is the earliest-stage sequencing data that remains.
Institutions
- Korea UniversitySeoul, Seoul
Categories
Funders
- National Research Foundation of KoreaMinistry of Science and ICTDaejeon, DaejeonGrant ID: RS-2025-00517214