single-cell RNA-seq of multiple myeloma

Published: 22-03-2021| Version 1 | DOI: 10.17632/9vyfw8v59k.1
Contributors:
Shigeki Hirabayashi,
Kotaro Shirakawa,
Yoshihito Horisawa,
Tadahiko Matsumoto,
Hiroyuki Matsui,
Hiroyuki Yamazaki,
Anamaria Daniela Sarca,
Yasuhiro Kazuma,
Ryosuke Nomura,
Yoshinobu Konishi,
Suguru Takeuchi,
Emani Stanford,
Hideya Kawaji,
Yasuhiro Murakawa,
Akifumi Takaori-Kondo

Description

Preprocessed single-cell RNA-sequencing data of multiple myeloma. Bone marrow mononuclear cells from a patient with multiple myeloma were purified from bone marrow aspirates by standard density gradient centrifugation using Lympholyte-H (Cedarlane Laboratories Ltd.) and were cryopreserved in CELLBANKER 1 (Takara Bio Inc.). Frozen cells from the multiple myeloma patient were resuspended in RPMI-1640 medium with 10% FBS. The cDNA library was constructed from the sample using the Chromium Single Cell 3′ Library & Gel Bead Kit v2 (10x Genomics) according to the manufacturer’s instructions. The cDNA library was sequenced on an Illumina HiSeq 2500 platform. Alignment to human genome (GRCh38), de-duplication, filtering and generation of gene expression matrixes were performed with the Cell Ranger v3.0.2 (10x Genomics) according to the manufacturer’s instructions.

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