A study of The OsSAPK2–OsNAC4 module couples water stress signaling with cadmium accumulation in rice. Zhu et al.
Description
Dataset 1 provides the original and unprocessed data for figures 1–7 and supplemental figures 1–12 in the associated paper "The OsSAPK2–OsNAC4 Module Couples Water Stress Signaling with Cadmium Accumulation in Rice". It includes agronomic and cadmium (Cd) accumulation profiles of wild-type ZH11 and mutant lines under field and hydroponic conditions (Zhu et al. Figure 1, Figure S1); transcriptomic datasets, phylogenetic analyses, tissue expression patterns, nutrient deficiency responses, and OsNRAMP1 expression validation in various genetic backgrounds (Zhu et al. Figure 2, Figures S2, S3, S5, and S6); molecular interaction evidence including Y1H, transactivation, ChIP-qPCR, and EMSA assays (Zhu et al. Figure 2, Figure S4); protein-protein interaction and phosphorylation analyses between OsSAPK2 and OsNAC4/5, featuring Y2H, pull-down, LCI, Co-IP, BiFC, and Phos-tag assays (Zhu et al. Figure 3, Figure S7); genetic, physiological, and transcriptional characterization of osapk2 knockout lines, OsSAPK2 overexpressing lines, and response profiles to exogenous ABA (Zhu et al. Figure 4, Figures S8 and S9); cell-free protein degradation dynamics, subcellular localization, genetic interaction webs, and DNA-binding activity regulation mediated by OsSAPK2 phosphorylation (Zhu et al. Figure 5, Figure S10); comprehensive field agronomic traits, grain Cd content, and different water management regime evaluation of osnac4 knockout lines across multiple genetic backgrounds (Zhu et al. Figure 6, Figures S11 and S12); and the mathematical validation for the proposed working model (Zhu et al. Figure 7).
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Institutions
- Institute of Soil ScienceJiangsu, Nanjing