Data supporting “Effects of Drying Conditions on Phytochemical Composition, Antioxidant Activity, and UPLC-HRMS Profiling of Rhizophora mucronata Herbal Matcha-Like Powder”

Description

This dataset contains experimental data supporting a study on the effects of hot-air drying temperature and duration on the antioxidant activity and UPLC-HRMS metabolite profile of Rhizophora mucronata herbal matcha-like powder. 1. The drying experiment used a completely randomized 3 × 3 factorial design: - Drying temperatures: 60, 65, and 70 °C - Drying durations: 120, 135, and 150 min - Independent replicates: n = 3 per treatment combination - Technical replicate measurements were averaged within each independently processed sample before statistical analysis. 2. TREATMENT CODES A1B1 = 60 °C, 120 min A1B2 = 60 °C, 135 min A1B3 = 60 °C, 150 min A2B1 = 65 °C, 120 min A2B2 = 65 °C, 135 min A2B3 = 65 °C, 150 min A3B1 = 70 °C, 120 min A3B2 = 70 °C, 135 min A3B3 = 70 °C, 150 min 3. Rhizophora mucronata Matcha-like Antioxidant_DPPH_ABTS_FRAP_RawData.xlsx Replicate-level antioxidant assay data. DPPH IC50: µg mL^-1 ABTS IC50: µg mL^-1 FRAP: µmol TE mg^-1 DW 4. STATISTICAL ANALYSIS The drying experiment followed a completely randomized 3 × 3 factorial design. The effects of drying temperature, drying duration, and their interaction were evaluated using two-way ANOVA. Tukey’s honestly significant difference (HSD) test was used for post-hoc comparison at p < 0.05. 5. UPLC-HRMS INFORMATION UPLC system: Waters ACQUITY UPLC Column: HSS C18, 2.1 × 100 mm, 1.8 µm Flow rate: 0.20 mL min^-1 Injection volume: 5 µL Mass spectrometer: Xevo G2-XS QTOF Ionization: ESI positive mode Acquisition: MSE Mass range: m/z 50-1200 Representative putative annotations: Rhizophora Matcha-like UPLC-HRMSBPI.pdf Peak 1: Ferulic acid (RT 1.85) Peak 2: Caffeoylquinic acid isomer (RT4.64) Peak 3: 5,7,4′-Trihydroxyflavanone (RT 6.40) All three annotations should be treated as putative MSI Level 2 annotations. 6. DATA PROCESSING AND SOFTWARE GraphPad Prism 10.6.1: Waters MassLynx 4.2: UPLC-HRMS data processing and interpretation 7. FILE FORMAT AND REUSE Spreadsheet files are provided in .xlsx format. Raw data should remain unchanged. Processed or summary data should be clearly labeled as such. 8. CONTACT For questions concerning the dataset, contact: Prof. Albert Linton Charles National Pingtung University of Science and Technology alcharles@mail.npust.edu.tw or Dr. Yunita Eka Puspitasari Universitas Brawijaya yunita_ep@ub.ac.id 9. NOTES - Values reported as means ± SD in the manuscript are derived from three independently processed samples. - UPLC-HRMS compound assignments are putative MSI Level 2 annotations.

Institutions

• National Pingtung University of Science and Technology

  Taiwan, Pingtung City
• University of Brawijaya

  East Java, Malang

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