Intratympanic administration of human umbilical cord mesenchymal stem cell-derived exosomes after acoustic trauma: an experimental rat model
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Auditory Brainstem Response Threshold Data in a Rat Model of Acoustic Trauma at Baseline and Days 3, 7, and 21
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Auditory brainstem response (ABR) threshold data were obtained from an experimental rat model of acoustic trauma. Thirty-two male Sprague–Dawley rats were randomized into four groups: positive control (acoustic trauma, untreated), study (acoustic trauma + intratympanic exosome), negative control (intratympanic saline, no acoustic trauma), and exosome-only/toxicity control. Acoustic trauma was induced by exposing freely moving animals to 6–10 kHz noise at 120 dB SPL for 4 hours. In the treatment group, 30 µL of human umbilical cord mesenchymal stem cell-derived exosome suspension (5 × 10⁹ particles/mL) was administered intratympanically into the right middle ear on post-trauma day 1. ABR thresholds were measured bilaterally at 8, 12, 16, 24, and 32 kHz at baseline and on post-trauma days 3, 7, and 21 using a SmartEP evoked-potential system with high-frequency software (Intelligent Hearing Systems, Miami, FL, USA). Tone-burst stimuli were delivered through calibrated high-frequency transducers and insert earphones. Stimulus intensity was initially set at 80 dB SPL and reduced in 10-dB steps using a descending–ascending approach; the lowest intensity producing a reproducible waveform was recorded as the ABR threshold. Measurements were performed by a blinded assessor. The deposited dataset contains the recorded threshold values for each animal, ear, frequency, and time point. The original measurements were reorganized into a structured tabular format for data sharing; numerical ABR values were not altered and missing observations were not imputed. Four animals were excluded during follow-up, leaving 28 animals (n = 7 per group) for the final analysis. Statistical analyses in the associated study were performed in Python 3 using SciPy and pandas.