SLRC_LsPtx
Description
Sequence data for two different peroxinectins in salmon lice, Lepeophtheirus salmonis. Obtained antibody responses post vaccination. Data from challenge with infective stages of L salmonis in naive and vaccinated fish with counting of lice numbers at moveable stages. Data show that one of the peroxinectins are indispensable for lice development. Vaccinated fish have reduced numbers of sea lice attached post vaccination and challenge but not down to significant reduction.
Files
Steps to reproduce
Sequencing of two peroxinectins from sea lice (Lepeophtheirus salmonis), tested for importance of lice development through RNAi studies, gene cloned and used in expression vector for vaccination. Recombinant protein used for boost. Collected plasma to assess immune responses post vaccination and performed studies to correlate to reduction in lice numbers.