Source Data for Adhesin Als4112 Promotes Candida auris Skin Colonization Through Interactions with Keratinocytes and Extracellular Matrix Proteins
Description
This dataset contains the numerical source data supporting all major and supplementary figures associated with the above publication. The study investigated the role of the Candida auris adhesin ALS4112 in: Keratinocyte adherence, Host skin colonization, Extracellular matrix binding, Biofilm formation, Catheter colonization, Fungal virulence The source data are provided as an Excel workbook containing separate worksheets corresponding to individual figures and supplementary figures from the publication.
Files
Steps to reproduce
The source data are provided as an Excel workbook containing separate worksheets corresponding to individual figures and supplementary figures from the publication. Files Included in This Repository File 1 Filename: 41467_2025_60876_MOESM4_ESM.xlsx Description: Source numerical data used for generation of all figures and supplementary figures reported in the publication. Worksheet tabs correspond to individual figures, including: Figure 1B, Figure 2A-E, Figure 3A, 3C-E, Figure 4A, 4D, Figure 5C, Figure 6A, Figure 7B-E, Figure S1-S3 Experimental Overview Experimental approaches represented in the dataset include : Keratinocyte adherence assays, High-throughput insertion mutant screening, Gene deletion and complementation studies, Gene expression analyses (qRT-PCR), Extracellular matrix protein binding assays, Murine skin colonization models, Murine systemic infection studies, Ex vivo human skin colonization studies, Biofilm formation assays, Catheter colonization experiments. Detailed methodology is published in the Zhao G, et al. Nature Communications (2025). Data Processing Raw measurements were collected from biological and technical replicates. Statistical analyses were performed as described in the associated publication. Summary metrics contained within the workbook may include: Means, Standard deviations (SD), Standard errors of the mean (SEM), P values, Z-scores, Relative gene expression values, CFU measurements, Software The data can be opened using Microsoft Excel, LibreOffice Calc, Google Sheets. Analyses and figure generation were performed using: GraphPad Prism, Microsoft Excel, Fiji/ImageJ, CellProfiler Related Resources Publication: Zhao G, et al. Nature Communications (2025). DOI: 10.1038/s41467-025-60876-1 Sequencing Data: NCBI BioProject: PRJNA1263008 License The dataset may be shared and reused provided appropriate citation is given to the associated publication and data repository record.
Institutions
- The Lundquist InstituteCalifornia, Torrance