A dataset of clinical parameters of infertile women undergoing IVF, including FSHR rs6166 and LHCGR rs2293275 genotypes
Description
A dataset of clinical parameters of infertile women undergoing through ovarian stimulation for assisted reproductive technologies in Rostov-on-Don, Russia. A short gonadotropin-releasing hormone antagonist protocol was used for ovarian stimulation. Early-follicular-phase follicle-stimulating hormone, luteinizing hormone, anti-mullerian hormone and progesterone levels were assayed in serum on 3-5-th menstrual cycle day 5-6 cycles before ovarian stimulation, with “Access hFSH”, “Access hLH”, “Access AMH” and “Access Progesterone” kits using Automatic Immunochemical Analyzer “Access 2” (Beckman Coulter, USA). Genotyping of the FSHR rs6166 variant was also carried out applying RFLP analysis using restrictase BsrI and primers: 5´-TTTGTGGTCATCTGTGGCTGC-3´, 5´-CAAAGGCAAGGACTGAATTATCATT-3´ [11]. Genotyping of the LHCGR rs2293275 variant was carried out applying primers: forward 5´-GCAACAGCTCCGTAACCAAG-3´, reverse 5´-GTGAAAGCACAGTAAGGAAAGTGTA-3´, and reverse mut 5´-TGAAAGCACAGTAAGGAAAGTGCG-3´. PCR products were detected by horizontal electrophoresis in 2% agarose gel. Results were validated by double genotyping.
Files
Institutions
Categories
Funding
Russian Science Foundation
23-15-00464