Diabetic Retinopathy E23K Dataset

Description

A case–control sample of 1,415 adults with T2DM was classified as diabetes without retinopathy (DWR; n=933), non-proliferative DR (NPDR; n=340), or PDR (n=141) based on ETDRS criteria, and compared with 1,389 normoglycemic controls. In this Middle Eastern population, KCNJ11 E23K functions as a modifier of progression to proliferative, vision-threatening DR rather than as an initiator of early retinopathy, with more pronounced effects under longer cumulative metabolic exposure.

Steps to reproduce

Genomic DNA for KCNJ11/Kir6.2 (rs5219) genotyping was isolated from peripheral venous blood samples of cases and controls using the QIAamp DNA Blood Mini Kit (Qiagen, Hilden, Germany). Allelic discrimination was performed on the Applied Biosystems StepOne Plus real-time PCR System using VIC/FAM-labelled TaqMan primers and probes, following the manufacturer’s protocols (Applied Biosystems; ThermoFisher Scientific, Waltham, MA); genotyping efficiency was 100%. Blinded replicate samples were included as quality controls and showed >99% concordance.

Institutions

• Tunis El Manar University

  Tunis Governorate, Tunis

Categories

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