Metabolomic Profiling and Identification of Potential Biomarkers in Borrelia garinii-Induced Lyme Disease raw datas
Description
A total of 120 SPF Balb/c mice were divided into eight groups (7, 15, 21, 30 days post infection) for both the experimental groups and the control groups, seven organ samples (heart, liver, spleen, lung, kidney, brain, and joint) were collected from each mice. Untargeted metabolomics sequencing was conducted at four different time points for seven organs from each mouse. Differential metabolites across seven organs were investigated through high-resolution gas chromatography-time of flight mass spectrometry (GC-TOF-MS). the heart, liver, spleen, lung, kidney, brain, and joint raw datas.
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In order to validate the quality of the sample and determine the optimal metabolite extraction and detection method for GC-TOF-MS analysis, a preliminary experiment was conducted. This experiment included an experimental group, comprising a randomly selected single sample, and a control group, which used a simple internal standard. In the formal experiment, the stability of the system was assessed by the retention time (R.T., measured in minutes) of the internal standard (L-2-chlorophenylalanine). There are two points that need special mention: 1) For the GC-Quad FiehnLib library, we have named derivatives by increasing numbers according to retention index, e.g. serine 1, serine 2 and serine 3 (for the derivatives with no one or two TMS-groups derivatizing the primary amino group). 2) The LECO/Fiehn metabolomics library was used to identify the compounds. It will give a similarity value for the compound identification accuracy. If the similarity is ＞ 700, we can think the metabolite identification is reliable. If the similarity is ＜ 200, the library will only use “analyte” for the compound name. If the similarity is between 200 and 700, the compound name is putative annotation. In our study, similarity > 700 was selected as the compound (metabolite).
Institutions
- Chinese Academy of Agricultural Sciences Lanzhou Institute of Husbandry and Pharmaceutical SciencesGansu, Lanzhou