Mass profiles of the TB isolates (JMSACL-D-25-00055)
Description
The .zip file contains the mass profiles tested in our study (JMSACL-D-25-00055). The unzip password is "JMSACL-D-25-00055." The dataset does not contain confidential or personal information for patients.
Files
Steps to reproduce
Please see our materials and methods: "TD-ESI/MS analyses For each testing isolate, mycobacterial colonies were scraped from Middlebrook 7H11 agar plates and suspended in 400 μL of 1× PBS. Each bacterial suspension was centrifuged, and the pellet was resuspended using chloroform-methanol (2:1, v/v) to extract the microbial lipids [34], and then transferred to a new vial for analysis. The crudely extracted sample was analyzed using thermal desorption–electrospray ionization/MS (TD-ESI/MS), which was set up with a metallic probe (60 mm long, 0.2 mm thickness, OD = 2.75 mm, ID = 1.5 mm; Ming Yuh Scientific Instruments, Tainan, Taiwan), a thermal desorption unit, and an electrospray ionization interface. This setup was coupled to a linear ion trap MS (LTQ XL Thermo Scientific, Waltham, MA, USA) operated in ESI mode, as previously described [35]. The sample (approximately 2 μL) was collected by dipping the metallic probe into the sample vial and then inserting the probe into the TD-ESI source for analysis. After each sample run, the metallic probe was exposed to high heat for five seconds using a butane torch (K-747, Changhua, Taiwan) to remove any residue from the previous sample. For the TD-ESI analysis, the desorption temperature was set at 280°C using a temperature controller (ANLY AT-502, Taipei, Taiwan), and the mass scan range was configured between m/z 400–1000. A metal tube (outer diameter = 15 μm and inner diameter = 5 μm) was attached to the heated oven to carry heated nitrogen gas to the desorption area, with the heated nitrogen flowing from the top of the TD unit to the ESI plume at a rate of 1 L/hr. A solution of 50% methanol (v/v) with 0.1% acetic acid (v/v) was used as the electrospray solution. A voltage of 4.0 kV was applied to the ESI capillary (outer diameter = 375 μm and inner diameter = 100 μm; Polymicro, Phoenix, AZ) to induce electrospray ionization via solution conduction. "
Institutions
- Mackay Medical College