Single-cell transcriptomics of the mouse retina and retinal organoids in X-linked retinoschisis

Published: 13 October 2025| Version 1 | DOI: 10.17632/cbct6bbctb.1
Contributors:
Shengru Mao, Xihao Sun, Zekai Cui, Jiansu Chen

Description

Retinas were harvested from Rs1 R102W point mutation mice at postnatal weeks 2 and 4 for single-cell transcriptomic analysis. Age-matched C57BL/6 mice served as controls, with a minimum of 10 retinas per group per time point. Retinal organoids were differentiated from induced pluripotent stem cells (iPSCs), which were reprogrammed from urinary cells of a patient with an Rs1 R102W point mutation. Retinal organoids derived from normal iPSCs were used as controls. Organoids were collected at day 50 for single-cell transcriptomic analysis, with a minimum of 20 organoids per group.

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Institutions

  • Aier Eye Hospital Group

Categories

Retina, Patient, Mouse Model, Single-Cell RNA Sequencing

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