Hodel et al Original Western blot data
Published: 22 May 2020| Version 2 | DOI: 10.17632/cdgs6fmpsn.2
Original Western blot data used for Figure S3 in Hodel et al. 50 ug of cell lysate from the indicated cell lines were separated via SDS-PAGE and transferred to nitrocellulose membrane using standard conditions. The blot was then physically cut into sections and stained with the indicated primary antibodies, inlcuding p53, P-p53, Mdm2, p21, PUMA and GAPDH as a loading control. Longer exposures are also provided for Mdm2 and p53.