Lipid analysis of sweet potato sucrose gradient (amyloplast subcellular fractionation)
Published: 17 May 2021| Version 1 | DOI: 10.17632/cg9xw9m7gc.1
Contributor:
Margit DrapalDescription
"5SwPot": Sweet potato roots (freeze-dried powder) were extracted from five different phenotypes (W-white, Y-yellow, O-orange, LP-yellow/purple, P-purple) and subjected to analysis by LC-MS/MS with a HILIC and C8 column. This allowed the separation of phospho- & galactosyl lipids and TAGs, respectively. Data was processed with Agilent Profinder. "Fractions": Sweet potato amyloplasts were extracted from two different phenotypes (O-orange, P-purple) and subcellular components separated with a sucrose gradient. The fractions were extracted with chloroform and analysed by LC-MS/MS with a HILIC and C8 column. This allowed the separation of phospho- & galactosyl lipids and TAGs, respectively. Data was processed with Agilent Profinder.
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Institutions
Royal Holloway University of London
Categories
Lipidomics