Solid Phase Condensates In Vitro Imaging Data
Description
Images of solid nucleoprotein condensates assembling over a greek island DNA fragment.
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Steps to reproduce
Protein aliquots were thawed immediately before each assay was performed. Full-length proteins were mixed together and diluted to a final concentration of 4uM in 20mM HEPES pH 7.4, 75mM NaCl, and 10% glycerol. DNA was added to a final concentration of 60nM. Components were mixed then transferred to a Superfrost Plus PreCleaned microscope slide (FisherBrand, Cat.12-550-15) with double stick tape to ensure more space between slide and coverslip (Corning, Cat. 2850-22). Slides were imaged after 5 minutes using an Inverted A1R laser scanning confocal microscope. Images were captured with differential interference contrast (DiC) imaging with and without fluorescence. Four images were taken per imaging condition and each condition was imaged in triplicate imaging sessions with independent protein samples.