Bioactivities of Polyphenols, Polysaccharides, and Oligosaccharides Derived from Two Wild West African Ganoderma Species
Description
This dataset has been updated to include only raw experimental data. Final tables and figures are available in related article. This dataset contains the raw experimental data associated with the above manuscript. Data are organized in a single excel file (Ganoderma species RawData.xlsx) with the following sheets: 1. Yield of Extracts – Raw weight and percentage yields of extracts obtained from Ganoderma enigmaticum and G. mbrekobenum (3 replicates) 2. Extract Types and Concentrations- List of extract types/fractions (Polyphenols, polysaccharides and oligosaccharides, showing their total phenolic and carbohydrate contents (3 replicates) 3. Antioxidant Activity- Percentage Inhibition of Different Concentrations of the extracts (25-100 µg/ml) (3 replicates) 4. Antibacterial activity showing percentage inhibition values and replicates data, mean and SD of Escherichia coli and Staphylococcus aureus 5. Anticancer activity – Percentage inhibition of cancer cell lines proliferation (HepG2, HCT116 and MDA-MB231 model cell lines) from XTT assay with replicates, mean and SD values 6. Sequences for Phylogenetic Analysis- Details of taxon, voucher number, Country of origin and GenBank Accession numbers of sequences downloaded from the GenBank used for phylogenetic analysis, including sequences used in the study and submitted to the NCBI data repository. File Formats • Ganoderma species RawData.xlsx (primary dataset, raw experimental values) How to Use • Open excel file and navigate between sheets for different data categories • Data are unprocessed, except for basic calculations (means and standard deviations)
Files
Steps to reproduce
This dataset was generated during a study investigating the bioactivities of polyphenolic, polysaccharide, and oligosaccharide fractions from two newly described West African Ganoderma species—G. enigmaticum and G. mbrekobenum. The following procedures were employed: 1. Sample Collection and Molecular Identification • Fruiting bodies of both species were collected from decaying wood in Lagos Nigeria • Dried specimens were pulverized for DNA extraction using the Norgen Fungal/Bacterial DNA Isolation Kit. • The ITS1-5.8S-ITS2 rDNA region was amplified by PCR using primers ITS1 and ITS4. • Amplicons were sequenced using the Sanger method. • Raw sequences were edited and assembled using Geneious Prime, and species identity was confirmed via BLAST searches (NCBI). 2. Extraction of Bioactive Fractions • Polyphenols were extracted using 70% acidified methanol (HCOOH-acidified), followed by rotary evaporation and filtration • Polysaccharides were obtained via hot water extraction, ethanol precipitation, and freeze-drying • Oligosaccharides were isolated by mild acid hydrolysis (1% formic acid) of polysaccharides and purified by ethanol fractionation. 3. Quantification of Phenolics and Carbohydrates • Total phenolic content was quantified by the Folin–Ciocalteu method using gallic acid as standard. • Total carbohydrate content was determined using the Phenol-sulfuric method with glucose as standard. • Absorbance was measured at appropriate wavelengths using the BioTek Multiskan Microplate Reader (Bio Rad). 4. Antioxidant Activity Assay • DPPH radical scavenging assay was conducted to evaluate antioxidant potential across various concentrations (25–100 mg/ml). • Absorbance was read at 517 nm using the microplate reader, and inhibition percentages were calculated. 5. Antibacterial Activity Assay • Broth microdilution assay was used to assess antibacterial activity against: o Escherichia coli O157:H7 (ATCC BAA-3162) o Methicillin-resistant Staphylococcus aureus (MRSA, ATCC 700968) • Half maximal inhibitory concentration (IC50) was computed using percentage inhibition of different concentrations of the extracts with the GraphPad Prism software 6. Anticancer Assay • The XTT Cell Proliferation Assay Kit (ATCC® 30-1011K™) was used on: o HepG2 (liver cancer) o HCT116 (colorectal cancer) o MDA-MB-231 (triple-negative breast cancer) • Extracts were tested at concentrations between 25–100 µg/ml. • IC₅₀ values were calculated from dose-response curves. 7. Software and Data Analysis • Sequence editing and alignment: Geneious Prime • Sequence validation: NCBI BLAST • Graphing and statistics: GraphPad Prism 10.5 and Microsoft Excel
Institutions
- North Carolina Agricultural and Technical State University