North Pacific spiny dogfish lactate metabolism

Description

Datasets are presented for analyses in Bouyoucos et al. (In Prep). See the 'metadata' text file for descriptions of individual .csv files and descriptions of column headings. All animals used in this study were collected with a permit from Fisheries and Oceans Canada (XR 264 2023), and all experiments were approved by the Bamfield Marine Sciences Centre Animal Care Committee (RS-22-10[R1]). The data in these datasets test two putative mechanisms underlying the hypothesis that active recovery accelerates lactate clearance using a shark as an experimental model. Pacific spiny dogfish (Squalus suckleyi) were collected from the wild for use in three experiments. First, dogfish were exposed to a stressor to cause an accumulation of lactate (air exposure) and were recovered in conditions that either restricted movement or allowed for movement (i.e., active recovery); plasma and tissue samples were taken for determination of lactate and corticosteroids. Second, dogfish were sacrificed and tissue explants were incubated in media containing lactate and corticosteroid receptor agonists/antagonists to stimulate or inhibit lactate flux. Third, dogfish were sacrificed and tissues were permeabilized to quantify mitochondrial respiration rates with lactate as an oxidative substrate. Together, these data support the conclusion that active recovery accelerates lactate clearance in dogfish.

Steps to reproduce

A detailed description of the methods appears in a manuscript that will be submitted for peer review. Code used to analyze the data will be made available from the contributing author upon request.

Institutions

• University of Manitoba

  Manitoba, Winnipeg
• Mount Allison University

  New Brunswick, Sackville
• Bamfield Marine Sciences Centre

  British Columbia, Ucluelet

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