Fluorescence EEM data from PAHs in cachaça supported on nylon membranes
Description
Fluorescence EEM spectra of cachaça extracts concentrated on nylon membranes.
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Steps to reproduce
For the calibration set, five calibration mixtures containing benzo[a]pyrene (BaP), dibenz[a,h]anthracene (DahA), benzo[b]fluoranthene (BbF), and benzo[g,h,i]perylene (BghiP) were prepared by diluting stock solutions in 38% ethanol to final concentrations ranging from 24 to 120 ng·L⁻¹ for BaP, DahA, and BghiP, and 45 to 225 ng·L⁻¹ for BbF. For the validation set, six commercially available unaged cachaça samples were spiked with three different PAH mixtures at concentrations ranging from 36-84 ng·L⁻¹ for BaP, DahA, and BghiP, and 68-158 ng·L⁻¹ for BbF. Sample preparation involved dilution to 38% ethanol followed by a two-stage nylon membrane (0.45 μm) filtration process using a custom positive pressure system. The procedure comprises two sequential filtration steps. Initially, 50 mL cachaça samples were filtered through a nylon membrane. This step retains both PAHs and matrix interferers, such as organic matter and particulates, which can compromise fluorescence spectral measurements. To address this issue, the used membrane underwent a solvent extraction process with 4.5 mL of acetonitrile. Furthermore, an additional 4.0 mL of ultrapure water w/ere also passed through the membrane. The filtrate was transferred to a 50 mL volumetric flask and completed to volume with ultrapure water, promoting selective elution of the PAHs. The filtrate was collected in a 50 mL flask and made up with ultrapure water. This solution was filtered using the filtration system and a new membrane. Following 40 minutes of drying at room temperature, fluorescence measurements were performed directly on the membranes using a Cary Eclipse spectrofluorometer with optimized parameters (excitation: 250-306 nm; emission: 380-480 nm; steps: 2 nm; excitation and emission monochromator slits: 10 and 2.5 nm of bandpass, respectively).
Institutions
- Universidade Federal da Paraiba