CBMN assessment of carotenoids in HepG2 cells

Published: 5 April 2023| Version 1 | DOI: 10.17632/d23kss55b7.1
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Description

This dataset contains micrographs of HepG2 cells exposed for 24h to the following carotenoids: 4.6 µM β-carotene, 40 µM trans-β-apo-8’-carotenal and 10 µM β-ionone 10 µM. Controls included cells grown only in culture medium, cells grown in solvent control (0.5% acetone) and cells grown in positive control 20 µM methyl methanesulfonate (MMS), a mutagenic compound. Cells were further incubated with cytochalalin B for a sufficient period of time to allow nuclear division while preventing cytokinesis.

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Steps to reproduce

In 6-well plates, 5 × 10(5) HepG2 cells were seeded per well. After 24h, complete DMEM medium (10% FBS) was replaced with medium containing one of the following: - 40 µM trans-β-apo-8’-carotenal; - 4.6 µM β-carotene; - 10 µM β-ionone; - 0.5 % acetone (v/v) - 20 µM methyl methanesulfonate - control (complete DMEM only) After 24h, the medium was replaced with medium containing 3 µg/mL cytochalasin B, to prevent cytokinesis. After 30h, cells were washed with PBS and detached with 0.25% trypsin - 1 mM EDTQA solution. Cells in suspension, 5 × 10(3), were centrifuged and spotted on glass slides. After methanol fixation (10 min) and drying, cells were stained sequentially with Eosin Y dye and Azur B from Hemacolor staining kit, according to the kit's instructions.

Institutions

Universidade do Porto Faculdade de Farmacia, REQUIMTE LAQV Porto

Categories

In Vitro Toxicology

Funding

Ministry of Science Technology and Higher Education

UID/QUI/50006/2019

Programa Operacional Temático Factores de Competitividade

POCI-01-0145-FEDER-016530

Programa Operacional Temático Factores de Competitividade

POCI-01-0145-FEDER-029253

Fundação para a Ciência e Tecnologia

PTDC/QEQ-QAN/1742/2014

Fundação para a Ciência e Tecnologia

PTDC/NAN-MAT/29248/2017

Fundação para a Ciência e Tecnologia

SFRH/BPD/74868/2010

Licence