RILI patients' metagenome-DNA6G-RFB-rawdata
Description
Human fecal samples were collected from 25 RILI+ patients and 22 RILI- patients for metagenomic sequencing. Microbial genomic DNA was extracted from fecal samples using a commercial DNA extraction kit according to the manufacturer’s instructions. The concentration and purity of extracted DNA were assessed before library construction. For each sample, qualified DNA was used for sequencing library preparation. DNA was fragmented to the appropriate size, followed by end repair, A-tailing, and ligation with Illumina sequencing adapters. The ligated products were amplified by PCR and purified using magnetic beads. Library quality was assessed by Qubit fluorometric quantification and Agilent Bioanalyzer analysis. Qualified libraries were then sequenced on an Illumina high-throughput sequencing platform to generate paired-end reads. Raw sequencing reads were quality-filtered to remove low-quality reads, adapter contamination, and host-derived sequences. The remaining high-quality reads were used for downstream metagenomic analysis. Statistical analyses and visualization were performed using R packages including vegan, ggplot2, and pheatmap, together with LEfSe where applicable. The grou