Physiological Metabolic Responses and Survival Rate of Hylurgus ligniperda to High Temperature Stress-new
Description
We investigated the effects of heat-induced mortality and stress on the physiological metabolism of Hylurgus ligniperda under various high-temperature conditions. Specifically, we recorded the survival of H ligniperda adults and larvae under different high temperature treatments, and the changes in water content, fat, glycogen, protein, sorbitol, trehalose, and glycerol in response to heat stress.
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We employed chloroform-methanol extraction to measure the lipid content of Hylurgus ligniperda adults and determined the content of glycerol, trehalose, and sorbitol. The glycogen, BCA protein, sorbitol, glycerol, and trehalose kits (Shanghai Enzyme Linked Biotechnology Co., Ltd., China) were used to measure the respective contents of glycogen, protein, sorbitol, glycerol, and trehalose.
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Funding
National Key Research and Development Program of China
2021YFD1400300