Differential analysis of pulmonary smooth muscle cells vs aortic smooth muscle cells

Description

This dataset provides paired transcriptomic (RNA-seq) and proteomic profiling of vascular smooth muscle cells (VSMCs) from pulmonary artery and aorta under hypertensive and normotensive conditions in rats, aiming to identify tissue-specific molecular responses to hypertension. Four groups (n=3 each): NPA (normal pulmonary artery, group A), HPA (hypertensive pulmonary artery, group B), NAA (normal aortic artery, group C), HAA (hypertensive aortic artery, group D). Two comparisons: HPA vs NPA (B vs A) and HAA vs NAA (D vs C). Transcriptomics folder: RNAseq_inputdata_HPAvsNPA_HAAvsNAA.xlsx: raw gene expression matrix (12 samples). data_RNAseq_HPAvsNPA_HAAvsNAA_p0.05.csv: 1,429 differentially expressed genes (p<0.05) with fold change, log2FC, p-values, and regulation labels. Up_Down_data_RNAseq_HPAvsNPA_HAAvsNAA_p0.05.csv: 38 genes with divergent/concordant regulation between tissues (L2/L3 classified). RNAseq_HPAvsNPA_HAAvsNAA_p0.05.pdf: volcano plots. Proteomics folder: protein inputdata_BvsA_DvsC.xlsx: raw quantification for 1,854 proteins across 12 samples, with UniProt accessions and annotations. data_protein_ABCD_p0.05.csv: 40 differentially expressed proteins (p<0.05) with fold change, log2FC, p-values, and regulation labels. Up_Down_data_protein_ABCD_p0.05.csv: 5 proteins with divergent regulation between tissues (L2/L3 classified). protein_BvsA_DvsC_p0.05.pdf: volcano plots. Species: Rattus norvegicus. Threshold: p<0.05; L2 = up in pulmonary/down in aorta, L3 = down in pulmonary/up in aorta. This dataset supports the associated article in Vascular Pharmacology.

Institutions

• Northwest University

  Shaanxi, Xi'an

Categories

Funders

• National Natural Science Foundation of China

  Beijing, Beijing

  Grant ID: 82070054
• Shaanxi Science and Technology Department

  Ministry of Science and Technology of the People's Republic of China

  Shaanxi, Xi'an

  Grant ID: 25JE032

Licence