A SNP in a Steroidogenic Enzyme Is Associated with Phenotypic Sex in Seriola Fishes. Koyama et al.

Published: 22 May 2019| Version 1 | DOI: 10.17632/dhv3nj8n89.1
Contributors:
Takashi Koyama,
Masatoshi Nakamoto,
Kagayaki Morishima,
Takefumi Yamashita,
Kohei Sasaki,
Yosuke Kuruma,
Naoki Mizuno,
Moe Suzuki,
yoshiharu okada,
risa ieda,
Tsubasa Uchino,
Satoshi Tasumi,
Sho Hosoya,
Seiichi Uno,
Jiro Koyama,
Atsushi Toyoda,
Kiyoshi Kikuchi,
Takashi Sakamoto

Description

Figure M1. Amino acid sequence analyses of the Seriola HSD17B1s. (A) Neighbor-Joining full phylogenetic trees of short-chain dehydrogenase/reductases. Related to Figure 2A in the main text. Numbers on branches represent percentage bootstrap values (1,000 replications). GenBank accession numbers of each branch are shown in Table M14. (B) Maximum-Likelihood full phylogenetic trees of short-chain dehydrogenase/reductases. Related to Figure 2A in the main text. Numbers on branches represent percentage bootstrap values (1,000 replications). GenBank accession numbers of each branch are shown in Table M14. (C) Whole multiple alignment of HSD17B1 protein sequences. Related to Figure 2B in the main text. Dots indicate amino acids identical to those of human HSD17B1. Blue, red, grey, and green boxes indicate the catalytic centers, cofactor binding motif, structurally conserved residues, and the motif for the determination of reaction direction, respectively. Glutamic acid residues specific to amberjack and yellowtail Z-linked alleles (HSD17B1ZGlu) are shown in bold. The lysine residue encoded by the Z-linked minor allele in amberjack (Amberjack HSD17B1ZLys) is also shown in bold. Positions at the top of the alignment are same as in Figure 2B in the main text. Tables M1–M14. Additional supplementary tables not included in the main text. Titles are at the top of each table.

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Institutions

Tokyo Daigaku, Kokuritsu Idengaku Kenkyujo, Nippon Suisan Kabushiki Kaisha, Tokyo Kaiyo Daigaku, Kagoshima Daigaku

Categories

Genetics, Genomics, Animal Reproduction, Animal Sex Determination, DNA Sequencing, RNA Sequencing, Steroidogenesis, Molecular Dynamics, Aquatic Biology, Evolution of Sex

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