Kalincuang of Uncaria gambir as antihypertensive and antioxidant
Description
This dataset accompanies the manuscript titled “Antihypertensive and Antioxidant Potential of ‘Kalincuang’, a Liquid By-product of Gambier (Uncaria gambir), in a Rat Model of Hypertension.” It contains supporting data from a preclinical study investigating the effects of freeze-dried Kalincuang on blood pressure regulation and oxidative stress in a rat model of induced hypertension. The dataset includes information on the preparation and standardization of Kalincuang, including its phenolic and catechin content as well as antioxidant capacity assessed via DPPH assay. Experimental data encompass hemodynamic parameters such as systolic and diastolic blood pressure, mean arterial pressure, heart rate, pulse pressure, blood flow, and blood volume, recorded at multiple time points during treatment. Biochemical data on plasma nitric oxide and malondialdehyde levels are also included to assess oxidative status. Rats were divided into groups receiving various doses of Kalincuang, alongside control groups treated with either captopril or vehicle. Statistical analyses are provided to evaluate the effects of treatment over time and across doses
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Steps to reproduce
To reproduce the findings in this study, begin by sourcing Kalincuang—the liquid by-product of Uncaria gambir processing—and subject it to filtration and freeze-drying. Standardize the dried extract by evaluating its physicochemical properties and antioxidant activity using a DPPH assay. Prepare male Wistar Kyoto rats and induce hypertension through oral administration of prednisone and a high-salt solution over 14 days, followed by a single intraperitoneal injection of L-NAME. Group the hypertensive rats and administer Kalincuang at varied doses, with captopril and vehicle as controls. Record hemodynamic parameters at defined time points using a non-invasive tail-cuff system. At the end of the treatment period, collect blood samples, isolate plasma, and measure nitric oxide and malondialdehyde levels using appropriate assay kits. Perform statistical analysis using ANOVA to assess treatment effects. Detailed procedures, dosing schedules, and analytical protocols are outlined in the accompanying documentation and data files to ensure full reproducibility.
Institutions
- Universitas Andalas